应用16S rDNA序列探讨斑腿蝗科的单系性及其亚科的分类地位  

Monophyly and the taxonomic status of subfamilies of the Catantopidae based on 16S rDNA sequences

作　　者：刘殿锋[1] 蒋国芳[1] 时号[1] 孙正莉[1] 霍光明[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物资源技术重点实验室,南京210097

出　　处：《昆虫学报》2005年第5期759-769,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目(30160015)

摘　　要：本文测定了斑腿蝗科10亚科20种蝗虫和其他蝗科3种蝗虫的线粒体16SrDNA部分序列,并从GenBank中下载了15种蝗亚目昆虫的16SrRNA基因相应序列片段。比对后的序列长度是397bp,其中有196个变异位点,157个简约信息位点,A+T平均含量为71.7%,C+G平均含量为28.3%。以序列差异比值为横坐标,以碱基转换数和颠换数为纵坐标作散点图,结果表明颠换多于转换,且随着差异程度的增加,转换明显出现了饱和。以蚱总科的日本蚱Tetrixjaponica和卡尖顶蚱Teredoruscarmichaeli作外群,用ME、等权MP、加权MP及贝叶斯法重建系统发生树。分子系统树表明,斑腿蝗科并非是一单系群,该科的切翅蝗亚科与稻蝗亚科也均不是一单系群;卵翅蝗、伪稻蝗和稻蝗三者有很近的亲缘关系;支持将黑蝗亚科和秃蝗亚科合为一个亚科——秃蝗亚科;现行的稻蝗亚科并非一单系群,而是一多系群。分子系统学研究结果和传统的基于形态特征的斑腿蝗科的分类体系有很大的不同。Fragments of mtDNA 16S rRNA gene were sequenced for 20 individuals from 10 subfamilies of the Catantopidae and 3 individuals of other families of Acridoidea. The homologous sequences of 15 species of grasshoppers were downloaded from the GenBank data library. It was found that the length of the alignment fragments was 397 bp, including 196 variable sites and 157 parsimonious informative sites. In the fragments, the percentages of A＋T and C＋G were 71.7% and 28.3%, respectively. The numbers of transitions and transversions among pairwise comparisons of the fragments were respectively plotted against percentage sequence difference. The number of transversions was higher than that of transitions, and saturation of transitions was discovered with the increase of sequence difference in the plot. Phylogenies were reconstructed using Tetrix japonica and Teredorus carrnichaeli as outgroup with ME, equally weighted MP, weighted MP and Bayesian inference. The monophyly of Catantopidae is not supported by phylogenetic trees. Inside of Cantantopidae, neither Coptacrinae nor Oxyinae is supported as a monophyletic group. The genera Caryanda, Oxya and Pseudonym are closely related. Combining Podisminae and Melanoplinae into one subfamily Podisminae is supported by phylogenetic trees. The current Oxyinae is not a monophyletic group, and should be a polyphyletic group. The result of inferred phylogeny is very different from the classification scheme of Catantopidae based on morphology.

关 键 词：蝗亚目 斑腿蝗科 16S RDNA 单系性 系统发生 分类地位 

分 类 号：Q969[生物学—昆虫学]