T4病毒研究进展  

Advances in tetravirus research

作　　者：易福明[1] 张珈敏[1] 刘传凤[1] 胡远扬[1] 

机构地区：[1]武汉大学生命科学学院,昆虫病毒研究室,武汉430072

出　　处：《昆虫学报》2005年第5期792-798,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30170037)

摘　　要：T4病毒科由一类单股正链RNA病毒组成,分为松天蛾β样病毒属和松天蛾ω样病毒属.这2个属的病毒具有不同的基因组结构,β样病毒含单组分基因组,其结构蛋白由一亚基因组RNA表达;而ω样病毒含双组分基因组,2个RNA分子分别编码复制酶蛋白和结构蛋白.在T4病毒基因组RNA 3′端有类似tRNA的二级结构.ω样病毒壳蛋白的氨基酸序列一致性高达66%～86%,而β样病毒壳蛋白的氨基酸同源性则要低得多.在昆虫细胞中表达壳蛋白基因时都能形成病毒类似粒子.该文还介绍了T4病毒复制机理以及T4病毒与其他病毒的进化关系.The family Tetraviridae is comprised of （＋） ssRNA viruses and divided into the Nudaurelia β-like viruses and Nudaurelia w-like viruses. The viruses of these two genera have different genomic organization. The β-like viruses have monopartite genomes and use a subgenomic RNA to express the structural protein, while the β-like viruses have bipartite genomes composed of two unique RNA molecules, which encode the replicases and the structural proteins, respectively. There is a tRNA-like structure on the 3＇-termini of the tetravirus genomic RNAs. The coat proteins of the w-like viruses show 66%-86% amino acid sequence identities, while the overall homologies between the coat proteins of the β-like viruses are much lower. When the coat protein ORFs of tetraviruses are expressed in insect cells, they are all able to form virus-like particles. The paper also reviewed advances on replication mechanism of tetrviruses and the evolutionary relationship among tetraviruses and others.

关 键 词：昆虫 T4病毒 基因组结构 tRNA类似结构 系统发育 

分 类 号：Q966[生物学—昆虫学]