结香花挥发油化学成分的气质联用分析  

GC-MS Analysis of Essential Oil Components from Flowers of Edgeworthia chrysantha Lindl.

作　　者：曹姣仙[1] 陈云龙[1] 傅承新[1] 吴平[1] 

机构地区：[1]浙江大学思源天然药物与生物毒素研究中心,杭州310058

出　　处：《药物分析杂志》2005年第10期1211-1214,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:用气相色谱-质谱联用法对结香花的挥发油进行化学成分分析。方法:使用乙醚萃取浓缩、水蒸气蒸馏的方法获得结香花挥发油,利用气相色谱-质谱联用法分离并分析其化学成分,采用 CC 峰面积归一化法定量。色谱条件:石英毛细管柱:HP-5(30 m×0.25 mm×0.25μm),载气为氦气,流速为1 mL·min^(-1)。程序升温从30℃(保留5 min)开始以8℃·min^(-1)升到120℃,再20℃·min^(-1),升至240℃,保留10 min。结果:鉴定出61种成分,共占挥发油总量的60.14%,其特有成分有1个含氮化合物、癸醚、7-溴甲基-7-十五烯和十六烷叔硫醇等。结论:本方法稳定可靠,重现性好,适用于结香花挥发油的化学成分分析。Objective： The chemical components of essential oil from the flowers of Edgeworthia chrysantha Lindl. were analyzed by GC - MS in this paper. Methods：The volatile components was extracted by diethyl ether, then isolated by steam distillation method. Their chemical compositions and relative contents were analyzed by GC - MS with HP -55% phenyl methyl siloxane capillary column（30 m ×0.25 mm ×0.25 μm）. The carrying gas was helium, the flow rate was 1 mL · min^- 1 and the temperature was in rate of 8 ℃ ·min^-1 from 30 ℃ （hold 5 min） to 120 ℃ ,then in rate of 20 ℃ · min^-1 from 120 ℃ to 240 ℃ （kept 10 rain）. Results： Sixty-one volatile compounds of Flos Edgeworthlae were identified, which comprised 60. 14% of volatile fraction. One nitrogenous configurations ; decane, 1,1 ＇- oxybis - ;7 - pentadecene 7 - bromomethyl - and tert - hexadecanethi- ol were especial components. Conclusion： The method is reliable, stabilize and reproducible, therefore this method can be applied to the analysis of the essential oil components, which was extracted from Flos Edgeworthlae components.

关 键 词：结香花 挥发油 化学成分 气相色谱-质谱联用 化学成分分析 气质联用分析 气相色谱-质谱联用法 结香 峰面积归一化法 石英毛细管柱 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]