二斑叶螨对梅岭霉素和溴虫腈的抗性选育及其解毒酶活力变化  

Resistance selection by meilingmycin and chlorfenapyr and activity changes of detoxicated enzymes in Tetranychus urticae

作　　者：李瑞娟[1] 王开运[1] 夏晓明[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018

出　　处：《植物保护学报》2005年第3期309-313,共5页Journal of Plant Protection

基　　金："十五"国家科技攻关项目(2001BA509B08)

摘　　要：在温室内模拟田间药剂的选择压力,用梅岭霉素和溴虫腈对二斑叶螨 Tetranychus urticae 以浸渍法逐代处理,选育其抗性种群,评价其抗性风险性。结果表明,选育至12代,对梅岭霉素抗性增长到9.2倍,对溴虫腈抗性增长到10.0倍。酶抑制剂和离体酶活性的测定结果表明,二斑叶螨对梅岭霉素的抗性可能与羧酸酯酶、多功能氧化酶解毒作用的增强和药剂对害螨体壁穿透性降低有关;而药剂对害螨体壁穿透性降低和谷胱甘肽-S-转移酶及酯酶代谢作用的增强可能是二斑叶螨对溴虫腈产生抗性的主要原因。In order to evaluate the resistance risk of meilingmycin and chlorfenapyr to Tetranychus urticae, resistance selection by slide slip method were carried out in laboratory. The related susceptible population of T. urticae was separately treated with meilingmycin and chlorfenapyr simulating field selection pressure in order to breeding its resistance. The results showed that the resistance of T. urticae had reached 9.2 folds to meilingmycin and 10 folds to chlorfenapyr after 12 generations treatment. The resistance mechanism was studied by detecting the activity changes of enzymic inhibitors and detoxicated enzymes. The rise of resistance to meilingmycin was because of the increased activities of carboxy lesterase, microsomal oxidase and decreased cuticular penetration. The resistance to chlorfenapyr was attributed to increased activities of glutathion-S-transferase and decreased cuticular penetration.

关 键 词：二斑叶螨 梅岭霉素 溴虫腈 抗性选育 解毒酶 活力变化 谷胱甘肽-S-转移酶 多功能氧化酶 羧酸酯酶 选择压力 

分 类 号：S482.52[农业科学—农药学]