葡多酚对小鼠肝细胞体内外增殖活性的影响  被引量:1

Effects of grape Procyanidins on proliferation of hepatocytes in vitro and vivo

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作  者:钟进义[1] 李蕾[1] 张丽华[1] 

机构地区:[1]青岛大学医学院,青岛266021

出  处:《中国公共卫生》2005年第11期1307-1308,共2页Chinese Journal of Public Health

基  金:国家自然科学基金(30271127)

摘  要:目的观察葡多酚(GPC)对正常肝细胞及受乙醇损伤的肝细胞增殖活性的影响。方法将小鼠正常肝细胞和乙醇引发损伤的肝细胞加入GPC培养,用四甲基偶氮噻唑蓝(MTT)法测定各组肝细胞增殖活性。将GPC经口给予正常小鼠和乙醇引发急性肝损伤的小鼠,取肝脏匀浆后培养3和16 h,用前述方法测定肝细胞增殖活性。结果体外实验的正常对照组和乙醇对照组细胞增殖活性分别为0.438±0.040和0.365±0.024;50 mg/L GPC保护组和50 mg/L GPC对照组则为0.541±0.026和0.603±0.060。体内实验的150 mg/L GPC保护组细胞增殖率较乙醇对照组升高0.095;150 mg/L GPC对照组则较正常对照组升高0.030。各组之间的差异均有统计学意义(P<0.05)。结论GPC能抑制乙醇对肝细胞增殖活性的损伤,并对正常肝细胞增殖活性有一定促进作用。Objective To study the effect of GPC on the proliferation of normal hepatoeytes and the cells damaged by ethanol. Methods ①Normal primary mouse hepatocytes and the cells damaged by ethanol were cuhrued with grape procyanidins(GPC)of different concentrations. Methyl thiazolyl tetrazolium(MTT) assay was used to analyze the cell proliferation. ②GPC was given to normal mouse and the ones with liver injuried by ethanol acutively through mouth. Cell proliferations of 3 h and 16 h were analyzed by the same way as above. Results In vitro the proliferations of normal control and the ethanol group were 0.438±0.040 and 0.365±0.024 respectively. Those of the 50 mg/L GPC protective group and control group were 0.541±0.026 and 0.603±0.060 respectively. In vivo, the proliferation rates of the 150 mg/L GPC protective group were 0.095 higher than that of the ethanol one. That of the 150 mg/L GPC control group was 0.030 higher than that of the normal control one. All differences above were significand ( P 〈 0.05 ). Conclusion GPC can inhibit the damage of ethanol on proliferation of hepatocytes, and promote proliferation of normal cells.

关 键 词:葡多酚(GPC) 肝细胞 增殖活性 

分 类 号:R114[医药卫生—卫生毒理学]

 

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