己糖激酶法和葡萄糖氧化酶法测定血糖的比较  

Comparison of HK and GOD- PAP assays for the detection of Glucose

作　　者：谢细娜[1] 

机构地区：[1]揭阳市人民医院,广东揭阳522000

出　　处：《基层医学论坛》2005年第10期867-868,共2页The Medical Forum

摘　　要：目的了解己糖激酶(HK)法和葡萄糖氧化酶(GOD-PAP)法测定血糖的性能。方法分别对两种方法进行了精密度试验、线性评价及方法对比试验。结果①精密度:HK法批内CV为0.95%,日间CV为2.15%;GOD-PAP法批内CV为1.02%,日间CV为1.75%。②线性:HK法为0.1￣28.0mmol/L,GOD-PAP法为0.1￣22.0mmol/L。③方法对比试验:以HK法为比较方法(Y),GOD-PAP法为试验方法(X),血糖的回归方程式为Y=1.053X+0.015,r=0.998。结论两种方法检测血糖的精密度、线性及方法对比试验均符合临床要求,适用于血糖的常规和自动化分析。Objective To evaluate the performance characteristics of HK and GOD-PAP assays for the detection of Glucose. Methods Compared the two assays with precision test, linearity test and method comparison test respectively. Results ①The precision of ilK was within-run CV 0.95%, between-run CV 2.15%; The precision of GOD-PAP was within-run CV 1.02%, between-run CV 1.75%. ② The linearity of HK was 0.1～28.0mmol/L, The linearity of GOD-PAP was 0.1～22.0mmol/L.③Correlation between results obtained by HK method （Y） and GOD-PAP method （X） was Y=1.053X＋0.015. Conclusions The two methods are suitable for routine and automatic measurement of Glucose in serum.

关 键 词：葡萄糖 己糖激酶法 葡萄糖氧化酶法 测定 葡萄糖氧化酶 己糖激酶法 酶法测定 血糖 精密度试验 对比试验 线性评价 PAP法 回归方程式 

分 类 号：R446.1[医药卫生—诊断学]