柴胡止血液对人工性周期大鼠置器后子宫内膜内皮素和一氧化氮动态变化的影响  

Influence of Chai-hu Hemostatic on Dynamic Regular Patterns of Endothelin and Nitrogen Monoxidum in Uterus of Rats Inserted Intrauterine Device

作　　者：周宜[1] 陈钢[1] 沈映君[1] 郭际[1] 侯建业[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《成都中医药大学学报》2005年第3期1-5,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(批准号:A30171134)

摘　　要：目的研究柴胡止血液影响安放IUD后大鼠性周期内子宫组织内皮素(ET)和一氧化氮(NO)动态变化的规律,进一步明确ET和NO在IUD出血副反应中的作用,为临床治疗提供新的思路和方法。方法建立人工性周期的动物模型,并用阴道涂片的方法测定其阴道上皮细胞角化率,用ELISA法测定大鼠子宫组织血清雌激素、孕激素水平以对其作出评价。观测安放Cu-IUD大鼠在人工性周期的不同时相,子宫组织ET-1、NO含量的动态变化以及柴胡止血液对其产生的影响。结果创建人工性周期大鼠模型与正常组比较,雌、孕激素水平无明显差异。安放IUD后,ET-1在周期前期有所升高,而在后期有所降低。柴胡止血液能通过调节平衡作用来对抗安放IUD引起的变化。而安放IUD后整个周期NO的含量均增高,柴胡止血液能降低NO的分泌。结论创建大鼠人工性周期模型成功,安放IUD后,对大鼠子宫组织ET-1、NO的动态变化产生了影响,而柴胡止血液能对抗安放IUD所造成的影响。Objective：To seek for the dynamic regular patterns of endothelin（ET） and nitrogen monoxidum（NO）, after Chai - hu hemostatic affused in the rats whose uterus were located by IUD （ intrauterine device）, finding clearer mechanism of IUD side - effects in order to provide better ideas for clinical treatment. Method： Make an investigation after developing rats IUD models, and establishing manual estrous - cycle rats models and testing keratinocyte ratio of vagina epithelia, and detemaining estrogen and progesterone of rats＇ uterus organism blood serum; testing dynamic movement of ET- 1, NO of Cu - IUD rats＇ uterus organism in manual estrous - cycle and Chaihu hemostatic effects. Results： Comparing manual estrous - cycle Rats Models to Normal Models, there were not obvious differences in the level of estrogen and progesterone. After located IUD in rats＇ uterus, ET- 1 raised in the early cycle , but it lowed in the later cycle; After the IUD was located, NO in the whole of the cycle raised much than normal, so that, Chaihu hemostatic could low NO exudation. Conclusion： Established manua/estrous - cycle rots models were effected; After we located IUD in rats＇ uterus, IUD made effects in the dynamic movements of ET- 1,NO of rats＇ uterus organism, however, Chaihu hemostatic could confront side- effects from the IUD.

关 键 词：宫内节育器 内皮素-1 一氧化氮 雌激素 孕激素 

分 类 号：R271.11[医药卫生—中医妇科学] R711.51[医药卫生—中医学]