rhKGF-2涂膜剂促进家兔皮肤烫伤愈合  

rhKGF-2 Liniment Accelerate Healing of Burn Wound in Rabbit

作　　者：缪云萍[1] 程敏[1] 叶小弟[1] 诸葛定娟[1] 王虎根[1] 郑高利[1] 

机构地区：[1]浙江省医学科学院药物研究所,杭州310013

出　　处：《浙江省医学科学院学报》2005年第3期20-23,共4页

基　　金：国家重大科技专项（863）“创新药物和中药现代化”专项基金资助（No：2004AA283141）

摘　　要：目的 研究rhKGF-2涂膜剂对皮肤深Ⅱ度烫伤的治疗作用。方法 用预热100℃的100g砝码在家兔背部脱毛部位烫8秒钟，形成4个直径约2．5cm的深Ⅱ度烫伤口，第2天开始每天伤口涂抹rhKGF-2涂膜剂150μl／伤口（30、15和7．5μg/伤口）1次，每4天描记伤口面积并计算伤口愈合率，烫伤后16天取伤口及其周围皮肤组织，石蜡包埋、切片、Masson’s三色法染色，MOTIC Advanced 3．2形态分析软件分析新生上皮细胞形成面积、厚度和迁移距离等。结果 rhKGF-2涂膜剂30μg／伤口和15州伤口对家兔皮肤深Ⅱ度烫伤的愈合均有明显的促进作用，能加快伤口愈合速度，促进上皮细胞再生，使新生上皮面积、平均厚度和移行距离明显增加。结论 rhKGF-2涂膜剂能明显加快皮肤烫伤后上皮细胞的增生与迁移，加快烫伤皮肤的愈合过程。Objective To observe the effect of recombinant human keratinocyte growth factor-2 （rhKGF-2） liniment on the healing of deep Ⅱ degree bum wound. Methods The back skin of each rabbit was burned for 8 seconds by 100℃ weights with diameter 2.5cm to become four deep Ⅱ degree burn wounds. The experimental animals were divided into 6 groups：the wound surfaces were daubed with saline solution as negative control group, liniment vehicle in vehicle group, rhKGF-2 solution in positive group, and rhKGF-2 liniments in the rest three groups （200, 100 and 50 μg/ml） daily for 15 days. The wound areas were measured at 4^th d, 8^th d, 12^th d and 16^th d. On the 16^th postinjury.Skin tissue biopsy specimens of the wound were taken. The area and thickness of neoepithelia tissue and the migrated distances of neoepidermal cells were detected with MOTIC Advanced 3.2 morphological analysis software after Masson＇s Trichrome staining. Results rhKGF-2 liniment accelerated deep Ⅱ degree burn wound in rabbit,accelerated the healing rate of wound, improved reepidermidation and increased the migrated distance, average area and thickness of neoepithelium. Conclusion These results showed that rhKGF-2 liniment could significantly accelerate wound healing in rabbit in a dose-dependently manner.

关 键 词：重组人角质细胞生长因子 深Ⅱ度烫伤 创面愈合 rhKGF-2 烫伤愈合 涂膜剂 兔皮肤 皮肤深Ⅱ度烫伤 伤口愈合率 上皮细胞 

分 类 号：R644[医药卫生—外科学] R96[医药卫生—临床医学]