新扬州鸡血浆碱性磷酸酶（PAKP）变化规律研究  

The Change Rules of Plasma Alkaline Phosphatase in New Yangzhou Chickens

作　　者：陈国宏[1] 李碧春[1] 

机构地区：[1]扬州大学农学院动物科学系

出　　处：《西北农业学报》1996年第3期5-8,共4页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：用磷酸苯二钠法测定新扬州鸡血浆碱性磷酸酶（ＰＡＫＰ）活性。结果表明，家系间、性别间和同功酶类型间有显著差异；随日龄增加，ＰＡＫＰ活性逐渐下降，开产前回升；产蛋后４～５ｈ升至最高，之后，逐渐下降，２０ｈ降至最低；采食后，血浆碱性磷酸酶水平很高，６～１８ｈ降至较低水平，但相对稳定。聚丙稀酰胺凝胶电泳结果表明，该酶同功酶可分为Ｆ型（含有ＡＫＰＦ带）和Ｓ型（含有ＡＫＰｓ带）。通常情况下，Ｆ型酶和Ｓ型酶各具２条带，其相对含量随日龄变化明显。The plasma alkaline phosphatase (PAKP) levels were analysed by disodium phenyl orthophosphate method in new Yangzhou chickens. The results showed that difference among the families, sexes and isoenzyme types in the PAKP levels could be seen obviously. The levels of PAKP decreased gradually during the 6￣18 weeks of age, but increased before laying the first egg. Regular changes of PAKP levels were observed in the laying or hungry hens. It′s found that there were two zymograms through isoenzymes analysis with polyacrylamide gel electrophoresis, F type (AKP F band) and S type (AKP S band). In general, each type had 2 bands, and relative content changed obviously with days of age.

关 键 词：鸡 血浆 碱性磷酸酶 新扬州鸡 同功酶 

分 类 号：S831.1[农业科学—畜牧学]