辣椒茎尖培养脱毒研究  

Shoot Tip Culture for Elimination Virus in Chilli

作　　者：周俊[1] 肖英[1] 程秉铨[1] 彭湘儒 刘文朴[1] 

机构地区：[1]新疆农业科学院中心实验室乌鲁木齐市蔬菜所新疆

出　　处：《西北农业学报》1996年第3期23-26,共4页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：以新疆辣椒主栽品种“四平头”（带毒率较高）为试材，切取无菌苗生长点，以ＭＳ为基本培养基，在其上分别添加６－苄基腺嘌呤（６－ＢＡ）、吲哚乙酸（ＩＡＡ）、玉米素（ＺＴ）、细胞激动素（ＫＴ）、赤霉素（ＧＡ３）和萘乙酸（ＮＡＡ）、苯乙酸（ＰＡＡ）以及吲哚丁酸（ＩＢＡ）等生长调节剂进行培养。经筛选８０余种不同激素水平的培养基，其中以ＭＳ＋ＢＡ８＋ＩＡＡ０．０５～１．０较适宜茎尖的培养，ＭＳ＋ＫＴ０．２＋ＧＡ３０．５～５或ＭＳ＋ＺＴ８＋ＧＡ３２～３较适宜芽的伸长。将伸长１ｃｍ以上的芽转入ＭＳ＋ＩＢＡ２或ＭＳ无激素的培养基上利于根的生长，生根后移入土壤即可长成正常植株。成苗脱毒率达１００％。Segments dissected from the apex of germfree seedling of Xinjiang chilli, which is a staple cultivar named 'Sipingtou' in Xinjiang and also was detected that the rate of virus carried on their seeds was relatively high, were cultured on the basal medium of MS, supplemented with 6-BA, IAA, ZT, KT, GA 3, NAA, IBA and PAA etc. Over eighty kinds of culture medium with varying in growth regulator level were experimented. The results showed that MS+BA8+IAA 0.05￣1.0 was favourable for shoot tip culture. MS+KT 0.2+GA 3 0.5￣5 & MS+ZT8+GA 3 2￣3 were favourable for shoot elongation. MS+IBA 2 & MS were favourable for root growth. The rate of virusfree chilli produced by tissue culture in the study was 100%.

关 键 词：辣椒 茎尖培养 脱毒 植株再生 

分 类 号：S641.303.5[农业科学—蔬菜学]