铜(Ⅱ)离子的L-组氨酸络合物的超氧化物歧化酶样活性的研究  

Copper(II) Complex with L-histidine Spectroscopic Properties and Superoxide Dismutative Activity

作　　者：苏海洪[1] 王天佑[1] 包仕尧[1] 

机构地区：[1]苏州大学附属二院神经科学研究所,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2005年第5期813-815,共3页Suzhou University Journal of Medical Science

摘　　要：目的寻找具有超氧化物歧化酶样活性的人工合成物,进一步了解超氧化物歧化酶的化学活性结构和催化作用机制。方法合成铜(II)离子的L-组氨酸络合物,并测定它的分子量、可见光谱和电子自旋共振波谱等物理特性,用肾上腺素自氧化法测定它的超氧阴离子歧化的活性。结果分子量和铜的百分含量测定结果发现,它们属于CuL2型络合物。可见光谱和电子自旋共振波谱实验显示其结构为扭曲的平面四边形,反应动力学结果表明,它符合酶反应特征。根据电子自旋共振波谱实验得出了其和超氧化物歧化酶类似的作用机制。结论铜(II)离子的L-组氨酸络合物不仅具有和超氧化物歧化酶基本相似的活性结构,而且也能催化超氧阴离子的歧化反应。Purpose To study the copper（ Ⅱ ） complex with L-Histidine＇s spectropic properties and its superoxide dismutase activity, Methods The copper（ Ⅱ ） complex with L-histidine was synthesized. We used the spectroscopic meter and electronic spin resonance（ESR） tenique to study its spectroscopic properties; the epinephrine autooxidation was used to study its superoxidative activity. Results The properties of photospectroscopic and ESR spectra indicated that the chelating ligands around copper were expected to be a square planar tetrahedral arrangement. The effect of this complex being compared with superoxide dismutase（SOD） on the epinephrine autooxidation and nitroblue tetrazolium photoreduction suggested that this complex could catalyst the dismutation of superoxide as well as SOD, and they might have similar catalytic mechanism. Conclusion the copper（ Ⅱ ） complex with L-Histidine＇s has the similar activity center and structure with the SOD and showes the same activity as the SOD.

关 键 词：铜离子 络合物 超氧化物歧化酶 

分 类 号：R977.3[医药卫生—药品]