慢性肾衰患者血清中分子物质浓度变化及其对Na,K-ATP酶的抑制作用  

Concentration Variation of Middle Molecules in Sera of Chronic Renal Failure Patients and Inhibition for Na,K-ATPase

作　　者：杨眉[1] 储结根[1] 张建达[1] 袁直[1] 杨洪涛[2] 刘宏富[3] 赵菁丽 杨淑芬[3] 

机构地区：[1]南开大学高分子化学研究所,天津300071 [2]天津市中医学院第一附属医院,天津300193 [3]天津市天和医院,天津300050

出　　处：《高等学校化学学报》2005年第11期2066-2068,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目(批准号:G1999064707)资助

摘　　要：Sera from patients with different courses of chronic renal failure and healthy persons were separated by gel permeation chromatography,and the concentration of middle molecular fraction B were calculated.The concentration of fractions B was increasing with the aggravating of renal failure degree,which indicated that the concentration of fraction B was related to patients′ renal failure.Fractions B from uremic urine,uremic sera or normal urine were resolved into 10 to 17 sub-fractions by ion exchange chromatography.Sub-fractions from normal urine and from uremic sera were assayed for their inhibition for the activity of Na,K-ATPase.The results showed that the Na,K-ATPase activities of the samples with B-8 and B-9 were much lower than that of blank.It is indicated that B-8 and B-9 contained some compounds which could inhibit the activity of Na,K-ATPase.Sera from patients with different courses of chronic renal failure and healthy persons were separated by gel permeation chromatography, and the concentration of middle molecular fraction B were calculated. The concentration of fractions B was increasing with the aggravating of renal failure degree, which indicated that the concentration of fraction B was related to patients＇ renal failure. Fractions B from uremic urine, uremic sera or normal urine were resolved into 10 to 17 sub-fractions by ion exchange chromatography. Sub-fractions from normal urine and from uremic sera were assayed for their inhibition for the activity of Na, K-ATPase. The results showed that the Na, K-ATPase activities of the samples with B-8 and B-9 were much lower than that of blank. It is indicated that B-8 and B-9 contained some compounds which could inhibit the activity of Na, K-ATPase.

关 键 词：慢性肾衰 中分子 Na K—ATP酶 

分 类 号：Q592.1[生物学—生物化学] Q592.2