糖尿病骨质疏松大鼠骨代谢改变及双黄益骨方的干预作用(英文)  

Bone metabolism changes of the rats with diabetic osteoporosis and the intervention effect of Shuanghuang yigu recipe

作　　者：李赛美[1] 陈长青[1] 熊莉华[1] 杨艳红[2] 邓长青[1] 王志高[1] 张新亮[1] 朱艳芳[1] 莫伟[1] 

机构地区：[1]广州中医药大学附属第一医院糖尿病专科,广东省广州市510405 [2]广东省人民医院老年病科,广东省广州市510080

出　　处：《中国临床康复》2005年第35期187-189,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省中医药管理局课题(2000-2002年)(B100048)~~

摘　　要：背景：目前公认防治绝经期妇女骨质疏松症的有效方法是雌二醇，其副作用及其可能存在的风险制约了人们对药物的依从性；中医药以其药物天然、注重整体调节、多靶点效应等特点受到医学界关注。 目的：采用卵巢摘除术+链脲佐菌素复合方法建立绝经后糖尿病骨质疏松症大鼠模型，从骨代谢学角度探讨双黄益骨方对去卵巢糖尿病大鼠骨质巯松的治疗作用以及是否具有量效关系。 设计：完全随机设计，对照实验，多组问比较用方差分析，两两比较用t检验。 单位：广州中医药大学附属第一医院糖尿病专科。 材料：实验于2002-06／12在广州中医药大学中医疑难病重点实验室完成。选用6月龄清洁级雌性SD大鼠150只。将大鼠随机分为5组：假手术组，模型组，双黄益骨方23．00，5．75g／kg组及尼尔雌醇组，每组30只。方法：①去卵巢大鼠模型：将大鼠麻醉后，沿背部正中线向下做纵行切口，结扎并切断子宫角，摘除卵巢。假手术组不切除卵巢，余处理同去卵巢造模大鼠。②去卵巢糖尿病大鼠模型：去卵巢大鼠模型复制1周后，禁食12h，按45mg／kg腹腔内注射链脲佐菌素，分别于24和72h剪尾采血，用美国雅培公司快速微量血糖仪测血糖，血糖高于11．1mmol／L者。③去卵巢糖尿病骨质疏松症大鼠模型：去卵巢糖尿病大鼠成模后，饲养10周，测尿脱氧砒啶啉、生物力学指标，显著低于正常组即确定造模成功。④分组干预：双黄益骨方23．00，5．75g／kg组及尼尔雌醇组分别给相应药物23．00g／kg，5．75g／kg，0．33mg／kg，于去卵巢糖尿病骨质疏松症成模后给药。中药组加蒸馏水配制成1kg／L药液灌胃，早晚各1次；尼尔雌醇组加蒸馏水配制成0．15μ／L药液每4周灌胃1次。实验全程24周。⑤于实验结束时称量各组大鼠体质量，取大鼠尿及眼眶静脉窦血液，采用BACKGROUND： Estradiol is generally recognized to effectively prevent and treat osteoporosis in patients with menopause, but its side effect and possible risk restricts its application. Traditional Chinese medicine is noticed for its crude origin, concept of wholism and multiple target point effect. OBJECTIVE： To establish the model of rat with diabetic osteoporosis after menopause by the compound method of ovariectomy plus streptoxotocin,and to probe into the therapeutic effect of shuanghuang yigu recipe on the osteoporosis in rats with diabetics from bone metabolism angle and its dose-effect relationship. DESIGN： Completely randomized design and controlled experiment. SETTING： Department of Diabetics, First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine MATERIALS： The experiment was carried out in the key laboratory of Chinese Medicine Therapy of Difficult and Complicated Diseases,Guangzhou University of Traditional Chinese Medicine from June to December 2002.Totally 150 Sprague Dawley （SD） female rats of clean class were recruited and randomly divided into 5 group： namely, sham-operation,model group , shuanghuang yigu recipe 23.00,5.75 g/kg group and nilestriol group with 30 rats in each group . METHODS： ①Model of rats with ovariectomy： The rats were anesthetized with the antisepsis by iodine, the nick was cut along the middle line of the back and from up to bottom. Then the uterus angle was tied and cut off and the ovary was extirpated. The treatment of the sham-operation group was the same as model groups except that the ovary did not need to be extirpated. ② Diabetic rats model with overiectomy： One week after the ovariectomy, rats were fasted for 12 hours. Next step was to intraperitoneally inject streptozotocin （STZ） with the dosage of 45 mg per kilogram. The blood sample should be drawn at the time of 24 hours and 72 hours later separately by cutting tails. Then blood glucose was tested with blood glucose monitor of American Abbott Laboratories Ltd.

关 键 词：糖尿病/并发症 骨质疏松/病理生理学 汤剂 

分 类 号：R681.4[医药卫生—骨科学]