HPLC-FLD法测定人血浆中羧甲司坦的血药浓度  

Determination of Carbocisteine in Human Plasma by HPLC - FLD

作　　者：邓冲[1] 李锋武[2] 赵新锋[3] 赵欣[3] 祝忠民[3] 郑晓晖[3] 

机构地区：[1]陕西中医学院,陕西咸阳712083 [2]西安交通大学药学院,陕西西安710061 [3]西安交通大学中草药现代化研究工程中心,陕西西安710061

出　　处：《中国药业》2005年第10期67-68,共2页China Pharmaceuticals

基　　金：陕西省自然科学基金项目;项目编号:2000H10。

摘　　要：目的:建立一种高效、快速的液相色谱方法,测定人血浆中羧甲司坦的血药浓度。方法:OPA柱前自动进样器程序衍生,高效液相色谱-荧光检测法,外标法定量。结果:血浆中羧甲司坦线性范围是0.101 8-13.0 mg/L,检测限为0.035 46 mg/L,精密度试验RSD均小于7.2%,提取回收率均大于92.4%。结论:该方法简便、科学,易于操作。Objective： To establish a valid and fast high performance liquid method for determination of carbocisteine in human plasma. Methods： With the derived method use of OPA, the concentration of carbocisteine in human plasma is determined by external standard sample. Results： The linear range of carbocisteine is 0. 101 8 - 13, 0 mg/L, and the low detected limit is 0. 035 46 mg/L, and the RSD of precision are less than 7.2, and the recoveries are all more than 92, 4%. Conclusion： This method is simple, sensitive, and easy to operate.

关 键 词：羟甲司坦 衍生化 荧光检测法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R974.1[医药卫生—药学]