儿童福氏志贺菌TEM型β内酰胺酶耐药基因及其耐药性检测  

Detection of TEM-type β-lactamase gene and antimicrobial resistance in Shigella flexneri isolated from children

作　　者：俞蕙[1] 王晓红[1] 叶颖子[1] 薛健昌[2] 王岱明[1] 

机构地区：[1]复旦大学附属儿科医院传染科,上海200032 [2]复旦大学附属儿科医院细菌室,上海200032

出　　处：《中国抗感染化疗杂志》2005年第5期304-306,共3页Chinese Journal of Infection and Chemotherapy

摘　　要：目的了解儿童福氏志贺菌是否存在TEM基因及其发生率以及对耐药性的影响。方法对2004年6—11月从本院细菌性腹泻患儿粪便培养标本分离到的福氏志贺氏菌共51株,进行β内酰胺酶TEM全长基因PCR检测;用K-B法测定志贺菌对7种抗菌药物的敏感度。结果51株福氏志贺菌中17株检出TEM基因,阳性率33.3%;志贺菌对氨苄西林的耐药率高,其中TEM基因阳性志贺菌的耐药率为88.2%、TEM基因阴性志贺菌的耐药率为82.4%;对氨苄西林-舒巴坦也有一定的耐药,TEM基因阳性志贺菌、TEM基因阴性志贺菌的耐药率分别为41.2%、20.6%。结论从福氏志贺菌检出TEM型β内酰胺酶耐药基因,携带率为33.3%。Objective To investigate the TEM-type β-actamase gene in Shigella flexneri and its prevalence in children, as well as the impact of the gene on bacterial resistance. Methods Fifty-one strains of S. flexneri were isolated from faecal specimens of children with bacterial diarrhoea between June 2004 and November 2004 at Children＇s Hospital of Fudan University. TEM-type β- lactamase gcnc was detected by polymcrase chain reaction （PCR）. The susceptibility of S. flexneri strains to 7 antimicrobial agents was determined by K-B method. Results TEM-type β-lactmase gene was detected in 17 （33.3%, 17/51） strains of S. flexneri. The S. flexneri isolates were highly resistant to ampicillin. The resistance rate of TEM-gene positive S. flexneri was 88.2% , and that of TEM-genc negative S. flexneri was 82.4%. About 41.2% of TEM-gene positive S. flexneri and 20.6% of TEM gcne negative S. flexneri were resistant to ampicillin-sulbactam. Conclusions TEM gene was detected in 33.3 % of S.flexneri isolates from children.

关 键 词：福氏志贺菌 Β内酰胺酶 基因 耐药性 儿童 

分 类 号：R446.5[医药卫生—诊断学]