大豆耐盐性种质资源SSR遗传多样性及标记辅助鉴定  

Genetic Diversity Analysis and Marker Assisted Identification of Salt Tolerant Soybean by Using SSR Marker

作　　者：张海燕[1] 关荣霞[1] 李英慧[1] 王丽侠[1] 滦维江 常汝镇[1] 刘章雄[1] 邱丽娟[1] 

机构地区：[1]中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室

出　　处：《植物遗传资源学报》2005年第3期251-255,共5页Journal of Plant Genetic Resources

基　　金：国家"863"计划项目(2003AA207060);国家科技攻关项目(2004BA525B06)

摘　　要：本研究利用60对SSR引物对93份大豆1级耐盐种质资源和57份盐敏感种质资源(5级)进行分析,以确定耐盐种质资源和盐敏感种质资源的遗传多样性,以及耐盐相关标记在种质资源耐盐性鉴定中的利用程度。60个位点共检测出等位变异792个,平均13.2个,其中耐盐种质资源特有等位变异133个,盐敏感种质资源特有等位变异106个,但76.0%的等位变异频率低于0.10,仅有1.8%的等位变异出现频率高于0.40。耐盐种质资源多态性信息含量(PIC)平均为0.78(0.47～0.90),盐敏感种质资源PIC平均为0.80(0.46～0.94)。根据主成分分析,耐盐和盐敏感种质资源多样性分布规律相似。然而,与耐盐基因相关的一个SSR位点的等位变异分布频率在耐盐和盐敏感种质资源间无显著差异。根据UPGMA聚类分析结果,耐盐种质资源按生态区分为3个类群,类群Ⅰ和Ⅲ分别以东北春大豆和北方春大豆为主,类群Ⅱ为黄淮夏大豆和其他生态类型的混合群。A total of 150 soybean accessions, including 93 high tolerant （grade 0 or 1） and 57 sensi （grade 5） accessions were analyzed with 60 SSR markers in order assess the utilization degree of markers in salt tolerant germplasm an average of 13.2 alleles per locus with a total of 792 alleles at 60 ant germplasm specific and 106 were sensitive germplasm specific. 0.10 or less, and only 1.8% had frequency more than 0.40, indic to （i） measure the genetic diversity and evaluation. The result showed that there SSR loci, About 7 atlng a tive （ii） was in which 133 alleles were salt tolerant germplasm specific and 106 were sensitive germplasm specific. About 76% of the alleles had frequencies at 0.10 or less, and only 1.8% had frequency more than 0.40, indicating a high level of diversity. Mean polymorphic information content was 0.78 and 0.80 in salt tolerant and sensitive germplasm, ranged from 0.47 to 0.90 and 0.46 to 0.94 respectively. The result of PCA analysis showed that the genetic distributions were similar for salt tolerant and sensitive germplasm. However, the frequencies of alleles in SSR locus linked to a QTL of salt tolerance were not significantly different in them. According to the UPGMA cluster analysis, the salt tolerant germplasm was divided into three groups, among which group Ⅰ and group Ⅲ were mainly Northeast Spring soybean and North Spring soybean, group Ⅱ was a mixture of Huanghuai summer soybean and other ecotypes.

关 键 词：大豆 耐盐性 种质资源 SSR 遗传多样性 标记辅助鉴定 耐盐基因 

分 类 号：S565.1[农业科学—作物学]