柴胡对人乳头瘤病毒杀灭作用的实验研究  

Laboratory Study on Anti-human Papillomavirus Activity of Bupleurum Chinese

作　　者：李劲[1] 罗奎章[1] 林奕[1] 陈闻纳[2] 赖穗萍[3] 

机构地区：[1]广东省深圳市东湖医院皮肤科,深圳518020 [2]广州中山大学附属第一医院皮肤科,广州510080 [3]广州市东山区人民医院,广州510080

出　　处：《中国中西医结合皮肤性病学杂志》2005年第3期171-173,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的筛选柴胡抗人乳头瘤病毒(HPV)的有效成分。方法用溶媒(石油醚、乙醚、乙酸乙酯、正丁醇、乙醇、蒸馏水)对柴胡分别进行提取,应用荧光定量聚合酶链反应技术(FQ-PCR)对各提取物进行体外抗病毒药效学实验,检测离体尖锐湿疣皮损中HPV-DNA的扩增情况。结果HPV-DNA经柴胡水提物作用后,其PCR扩增检测为阴性,最低有效浓度为0.2g/mL,而用其他有机溶剂提取部分PCR扩增检测为阳性。结论柴胡的水提成分有抗人乳头瘤病毒作用。Objective To screen anti-human papillomavirus（HPV） effective fraction from Bupleurum Chinese. Methods Bupleurum Chinese was extracted with systematic solvents （petroleum benzin, ether, ethyl acetate, n-butanol, ethyl alcohol and distilled water） respectively. The fluoresence quantitative polymerase chain reaction （FQ-PCR） was applied in vitro pharmacodynamics for the extracts from Bupleurum Chinese. Than we examined the amplification of HPV-DNA in the lesions of the isolated condyloma acuminatum. Results After the water extracts of Bupleurum Chinese reacted with HPV-DNA, the PCR amplification showed negative with the minimum effective concentration 0.2 g/mL. But the other organic solvents extracts from Bupleurum Chinese were positive. Condusion The water extracts from Bupleurum Chinese can take effect on anti-human papillomavirus.

关 键 词：柴胡 尖锐湿疣 人乳头瘤病毒 聚合酶链反应技术 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]