猪传染性胃肠炎病毒纤突蛋白基因的修饰及原核表达的研究  

Study on modification of the transmissible gastroenteritis virus spike gene and prokaryotic expression of the modified gene

作　　者：钱刚[1] 冯力[1] 刘胜旺[2] 佟有恩[1] 童光志[2] 黄永芬[3] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 [2]中国农业科学院哈尔滨兽医研究所国家重点实验室,黑龙江哈尔滨150001 [3]哈尔滨师范大学生物系,黑龙江哈尔滨150080

出　　处：《东北农业大学学报》2005年第5期611-614,共4页Journal of Northeast Agricultural University

基　　金：兽医生物技术国家重点实验室开放基金

摘　　要：猪传染性胃肠炎病毒纤突蛋白是诱导猪产生特异性中和抗体的主要结构蛋白,包括A,B,C,D4个抗原决定位。为进一步生产TGEV植物转基因疫苗提供可操作性强、具免疫活性的外源基因,以猪传染性胃肠炎华毒弱株(TGEVH)的S基因为基础,扩增并连接4个抗原决定位相应的碱基序列,构建pPROEX-HTa原核表达载体,转化大肠杆菌诱导表达。结果表明,修饰后的S蛋白的表达量为19.8%,仍能和相应的血清发生抗原抗体反应。Transmissible Gastroenteritis Virus pigs produce neutralizing antibodies, including four （TGEV） Spike protein is the major protein which can make antigenic sites. Based on TGEV H spike gene, two fragments including all antigenic sites are jointed and cloned to plasmid pPROEX-HTa. Having been transferred into E. coli. （DH-5α）, it produces 19.8% spike protein when it induced by IPTG. At the same time, we find that the modified spike protein is capable of reaction to corresponding serous still. We intend to obtain foreign gene which is causative immunization and easy manipulation when we produce transgenic plant vaccine in future.

关 键 词：猪传染性胃肠炎病毒 S基因 修饰 原核表达 

分 类 号：Q343.11[生物学—遗传学] Q344.13