免疫介导再生障碍性贫血小鼠骨髓T细胞表面CD28、CTLA4水平检测及其意义  

Study on the Expression of CD28 and CTLA4 on T Lymphocytes of Bone Marrow in Immune-mediated Aplastic Anemia Mice

作　　者：刘振芳[1] 孙汉英[1] 刘文励[1] 罗小华[1] 何莉[1] 徐慧珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,湖北武汉430030

出　　处：《中国血液流变学杂志》2005年第3期341-343,共3页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目(No.30070326)

摘　　要：目的研究免疫介导再障小鼠骨髓T细胞CD28、CTLA4的表达及其意义。方法建立免疫介导再障小鼠模型,取再障和正常组小鼠骨髓单个核细胞(BMNC),在终浓度为15μg/mL植物血凝素(PHA)条件下培养,于0h和48h分别用双色免疫荧光标记流式细胞术对T细胞CD28、CTLA4的表达进行分析。结果PHA刺激培养48h后正常组和再障组T细胞CD28、CT-LA4的表达较刺激前均明显升高(P<0.01);而且再障组刺激培养前后T细胞CD28的表达均显著高于同期正常组(P<0.01),但CTLA4的表达与同期正常组相比虽略有升高,但差异无显著性(P>0.05)。结论再障T细胞激活及其激活潜能增强,免疫分子CD28、CTLA4在骨髓中的表达异常可能参与了再障T细胞的免疫功能紊乱。Objective To investigate the expression and significance of CD28 and CILA4 on T lymphocytes in bone marrow in aplastic anemia（AA） mice. Methods In vitro activation of T lymphocytes obtained from bone marrow in aplastic anemia mice by culture containing PHA（ 15ttg/mL）. The CD28 and CrLA4 expression on T lymphocytes at Oh and 48h after PHA exposure were analyzed by two color flow cytometry, Results The expression of CD28 and CTLA4 were significance elevated after PHA stimulation. In the group of AA mice, the expression of CD28 at Oh and 48h with PHA stimulation were higher than normal mice （ P 〈 0.01 ）, but the expression of CIIA4 at Oh and 48h with PHA stimu- lation were no statistical significance compare with normal mice（ P 〉 0.05）. Conclusion Increased activation and activated potentials of T lymphocytes in AA mice, abnormal expression of CD28 and CTLA4 maybe participate in immunological disorder of T lymphocytes in AA.

关 键 词：贫血 再生障碍性 T淋巴细胞 抗原 表面 CD28 CTLA4 

分 类 号：R556.5[医药卫生—血液循环系统疾病]