活化血小板表面膜糖蛋白的异位分布  

Redistribution of Platelet Membrane Glycoproteins on Activated Platelet Surface

作　　者：韩悦[1] 卢晓旭[1] 戴兰[1] 沈文红[1] 蔡秀英[2] 吴德沛[1] 

机构地区：[1]苏州大学附属第一医院血液科,江苏省血液研究所江苏苏州215006 [2]苏州大学附属第一医院神经内科,江苏苏州215006

出　　处：《中国血液流变学杂志》2005年第3期348-350,共3页Chinese Journal of Hemorheology

摘　　要：目的观察血小板活化后表面一些抗原决定簇的动态改变,探讨其在血栓性疾病研究中的意义.方法以凝血酶与腺苷二磷酸(ADP)分别在不同时间段(0～30min)诱导正常人血小板活化,应用流式细胞仪检测其血小板膜糖蛋白(GP)Ⅰb,GPⅢa及P-选择素的表达;同时用全血法测定脑梗死患者相应抗原的改变.结果凝血酶及ADP活化血小板后均可导致GPⅠb表达呈现先下降又逐渐回升的可逆性分布趋势,各时间段引起的GPⅠb改变在两者之间都具有显著差异;GPⅢa表达在活化后初期变化不明显,10min后呈现递增趋势;两种活化剂均可促使P-选择素显著升高,并维持于高水平.脑梗死患者GPⅠb表达减少,P-选择素的表达远高于对照组,GPⅢa无变化.结论凝血酶与ADP均能介导血小板活化,以时间依赖的方式促使GPⅠb与GPⅢa逆转,同时P-选择素向外释放,而血小板表面活化相关性抗原的改变是反映体内血小板活化的重要指标.Objective To investigate the redistribution of antigens on the platelet surface following activation, and assess its significance in the study of thrombosis disease. Methods By flowcytometry, the alterations of platelet membrane glycoproteins GP I b, GP m a and P-selectin were measured in normal subjects whose platelets were activated by thrombin and ADP at different time points（0 - 30minute） .The same antigens were also analyzed with whole blood assay in patients with cerebral infarction. Results A stable increase of P-selectin and reversible interalisation of GP I ba were obtained by both thrombin and ADP activation, and a different kinetic of GP I b redistribution was investigated for the two activators all over the time course （ P 〈 0.05）, while the platelet membrane GP m a was not significantly changed in the initial stage after platelet activation and increased by degrees 10 minutes later. Compared with those in normal group, there is an apparent increase of P-selectin and a lower expression of GP I b in the patients with cerebral infarction,while GPm a was not significantly changed in all the samples. Conclusion Either thrombin or ADP can mediate platelet activation, which leads to the redistribution of GPIb and GPm a in a time-dependent manner, together with the release of P-selectin. Meanwhile,the change of activation-dependent antigens on the platelet surface could be an important index reflecting the platelet activation in vivo.

关 键 词：血小板 糖蛋白类 脑梗死 凝血酶 流式细胞术 

分 类 号：R55[医药卫生—血液循环系统疾病]