纳米颗粒包裹CpG序列对猪副伤寒疫苗接种小鼠免疫应答的影响  

The Effect of Entrapment of CpG Sequence with Cationic PLG Nanoparticles on the Immune Responses of Mice to Pig Paratyphoid Vaccine

作　　者：武梅[1] 石林[2] 刘世贵[3] 李江凌[3] 吴凯源[3] 王丽焕[3] 沈翼[3] 刘昆[3] 郑勇[3] 张新申[1] 高荣[3] 

机构地区：[1]四川大学轻工技术与工程博士后流动站 [2]中科院成都生物研究所成都610062 [3]四川大学生物资源与生态环境教育部重点实验室

出　　处：《生物医学工程学杂志》2005年第5期975-979,共5页Journal of Biomedical Engineering

基　　金：国家教育部重点资助项目(00104);四川省"十五"重大科技攻关项目(01NG018-01)

摘　　要：本实验制备聚乙交酯丙交酯(PLG)纳米颗粒,比较了阳离子PLG纳米颗粒和脂质体作为包装分子包裹pUC18-CpG质粒对猪副伤寒疫苗接种小鼠体液IgG和特异抗体滴度、脾脏淋巴细胞增殖和白细胞介素-2诱生活性的影响。实验结果发现:与裸pUC18-CpG质粒接种小鼠比较,阳离子纳米颗粒包裹pUC18-CpG质粒能显著提高免疫小鼠IgG含量和特异抗沙门氏菌抗体滴度,增强淋巴细胞增殖活性及白细胞介素-2的诱生活性;同阳离子脂质体作为包装分子的细胞和体液免疫佐剂效应相似或较强。证明阳离子PLG纳米颗粒包装能显著提高裸CpG质粒的免疫增强活性。Cationic PLG nanoparticles and liposome were prepared and used as package molecules to pack up pUC18-CpG. The effects of the packed pUC18-CpG on the cellular and humoral immune responses were detected in the mice that were inoculated with pig paratyphoid vaccine. The results showed that compared with the control, the amount of IgG and the titre of specific antibody were significantly increased in the sera of mice immunized with the CpG plasmid entrapped by cationic PLG nanoparticles; the proliferation and induced IL-2 bioactivity of lymphocytes were significantly enhanced in the spleen of the immunized mice; the stimulatory effect of cationic PLG nanoparticles was similar to or stronger than that of cationic liposome. These indicated that cationic PLG nanoparticle could be employed as an effective package molecule to promote the immunostimulatory effect of pUC18-CpG.

关 键 词：小鼠 副伤寒疫苗 CPG序列 阳离子PLG纳米颗粒 免疫应答 纳米颗粒 CPG序列 免疫小鼠 疫苗接种 副伤寒 免疫应答 包裹 淋巴细胞增殖活性 白细胞介素-2 

分 类 号：S852.4[农业科学—基础兽医学]