淋巴细胞趋化因子受体mRNA在系统性红斑狼疮患者外周血单个核细胞的表达  

Expression of the c-class chemokine lymphotactin receptors XCR1 mRNA on peripheral blood mononuclear cells in patients with systemic lupus erythematosus

作　　者：李遇梅[1] 姚煦[1] 李安生[1] 钟连声[1] 陈志强[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所,南京210042

出　　处：《中华风湿病学杂志》2005年第10期582-585,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目(30170863);江苏省自然科学基金资助项目(BK2001195)

摘　　要：目的探讨淋巴细胞趋化因子受体(XCR1)mRNA在系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中的表达,及其与疾病的相关性。方法收集93例确诊的SLE患者和30名健康对照组,收集PBMC,提取RNA。应用反转录-聚合酶链反应(RT-PCR)方法检测研究对象XCR1mRNA表达水平。结果!"XCR1mRNA在PBMC中表达水平,患者组(1.57±0.84)与正常对照组(0.19±0.14),两者差异无统计学意义(P>0.05)。活动期(2.09±1.38)与对照组比较,两者差异有统计学意义(t=2.392,P=0.02);活动期与非活动期(0.31±0.24)比较,差异有统计学意义(t=3.091,P=0.003);非活动期与对照组比较,差异无统计学意义(P>0.05)。"#SLE患者组XCR1mRNA水平与疾病活动度评分(SLEDAI)关系:SLE患者组PBMC中XCR1mRNA表达水平与SLEDAI作直线相关性分析,XCR1mRNA水平与SLEDAI(r=0.540,t=5.445,P=0.000)呈正相关。"$SLE患者PBMC中XCR1mRNA表达与临床表现及实验室检查相关性:SLE抗SSA抗体阳性患者(17/68,1.4±1.2)比阴性患者(51/68,3.5±4.9),其XCR1mRNA表达水平降低,差异有统计学意义(t=2.78,P=0.007)。结论XCR1mRNA表达水平在PBMC中活动期SLE患者比非活动期及对照组增高,与疾病的活动性(SLEDAI)正相关,非活动期与对照组差异无统计学意义。SLE抗SSA抗体阳性患者比阴性患者,其XCR1mRNA表达水平降低,两组均较健康对照组增高。提示XCR1参与疾病的发展,与疾病的活动性相关,XCR1可能参与抗SSA抗体的形成过程,造成组织损伤。Objective Infiltrating lymphocytes have been demonstrated to play an important role in the tissue injury that occurs in systemic lupus erythematosus （SLE）. Inflammatory chemokines control lymphocyte traffic through their interaction with T cell chemokine receptors. In this study we assessed the expression of chemokine receptors XCR1 on peripheral blood mononuclear cell （PBMC） with active or inactive SLE. Methods Ninety-three definitive SLE patients and 30 healthy controls were enrolled. PBMC were separated from the peripheral blood specimen. Reverse tra＇nscription-polymerase chain reaction （RT-PCR） technique was applied to semi-quantitatively analyze the expression of ehemokine receptors （XCRI） in PBMC. Results ① The level of XCR1 mRNA expression was significantly increased in active patients with SLE as compared with controls, and showed significantly positive correlation with disease activity （SLEDA1）. ② The level of XCR1 mRNA expression had no difference between the inactive and controls. ③ XCR1 expression in patients with anti-SSA were significantly less than patients without anti-SSA. Conclusions The result suggests that XCR1 may be involved in the pathogenesis of SLE, and the expression level may be a good indicator for the disease activity of SLE.

关 键 词：受体 趋化因子 红斑狼疮 系统性 外周血单个核细胞 

分 类 号：R593.241[医药卫生—内科学]