敬钊缨毛蛛毒素-Ⅷ的分离纯化与鉴定  

Purification and Characterization of a Neurotoxic Peptide JZTX-Ⅷfrom the Ven om of the Spider Chilobrachys jingzhao

作　　者：胡喆[1] 王美迟[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,中国湖南长沙410081

出　　处：《生命科学研究》2005年第3期212-217,共6页Life Science Research

基　　金：国家自然科学基金资助项目(30170193)

摘　　要：从敬钊缨毛蛛(Chilobrachys jingzhao)粗毒中,通过阳离子交换色谱和反相高效液相色谱分离得到一种新的多肽神经毒素,命名为敬钊缨毛蛛毒素-Ⅷ(jingzhaotoxin-Ⅷ,JZTX-Ⅷ).经MALDI-TOF分析表明该多肽分子质量为4329.37D a,结合氨基酸序列分析和RACE的cDNA快速扩增法得到了敬钊缨毛蛛毒素-Ⅷ的氨基酸序列和全长cDNA,序列为LFECSFSCDIKKNGKPCKGSGEKKCSGGWRCKMNFCVKVC-OOH,其中6个半胱氨酸形成3对二硫键.敬钊缨毛蛛毒素-Ⅷ能阻断小鼠膈神经-膈肌标本的神经肌肉接头传递,经初步膜片钳实验分析,表明该多肽是一种钙离子通道抑制剂.A novel neurotoxic peptide, named JZTX-Ⅷ, has been purified through ion exchange chromatography and reverse phase HPLC from the venom of the spider Chilobraehys jingzhao. The molecular weight of the peptide was determined as 4 329.37 Da by mass spectrometry . The amino acid sequence is determined to be LFFCSFSCDIKKNGKPCKGSGEKKCSGGWRCKMNFCVKV-COOH, which has 39 amino acid residues containing 6 cysteine residues forming three disulfide bonds, the complete eDNA of JZTX-Ⅷ is also identified by a combination of automatic Edman degradation and RACE approach （Rapid Amplification of eDNA Ends）. JZTX-Ⅷ can block the neuromuscular transmission of the isolated mouse phrenic nerve-diaphragm preparation and block Ca^2＋ channel in DRG cells.

关 键 词：多肽神经毒素 敬钊缨毛蛛毒素-Ⅷ 敬钊缨毛蛛 

分 类 号：Q51[生物学—生物化学]