高效液相色谱法测定抗骨增生片中淫羊藿苷的含量  

Determination of Icariin in Kangguzengsheng Tablets by HPLC

作　　者：王兆华[1] 王淑琴[1] 弥宏[1] 张大军[1] 

机构地区：[1]吉林省中医中药研究院,长春130021

出　　处：《中国药师》2005年第10期829-830,共2页China Pharmacist

摘　　要：目的:应用高效液相色谱法测定抗骨增生片中淫羊藿苷的含量。方法:采用Shim-pack-C18柱(5 μm,150×4．6 mm), 乙腈-0．4％磷酸(29:71)为流动相,检测波长为270 nm,结果:线性范围为0．20-1．00 μg,r=0．999 6,平均回收率为 97．88％,RDS=1．13％。结论:该方法用于测定抗骨增生片中淫羊藿苷的含量,简便、准确、灵敏,可用于该制剂的质量控制。Objective： To determine the content of icariin in kangguzengsheng tablets by HPLC. Method： The Shimpack-C18 column（5 μm, 150 mm×4.6 mm）was used. The mobile phase was acetonitrile-0.4% phosphoric acid solution （29：71 ）. The detection wavelength was 270 nm. Result： Icariin showed a good linear relationship within a range of 0.20-1. 001μg（ r =0. 999 6）. The average recovery was 97.88% （RSD = 1.13% ）. Conclusion： The method is simple, accurate and sensitive. It can be used for quality control of the preparation.

关 键 词：高效液相色谱法 淫羊藿苷 抗骨增生片 

分 类 号：R927.2[医药卫生—药学]