反相高效液相色谱法测定人血浆中阿替洛尔的浓度  

Determination of Atenolol in Human Plasma by RP - HPLC

作　　者：谢赞[1] 蒋晔[1] 郝晓花[1] 王淑梅[2] 殷立新[2] 

机构地区：[1]河北医科大学药学院,石家庄050017 [2]河北医科大学第二医院药剂科

出　　处：《中国药师》2005年第10期832-834,共3页China Pharmacist

摘　　要：目的:建立反相高效液相色谱法测定血浆中阿替洛尔浓度。方法:以乙酸乙酯-异丙醇(8:1)为提取溶剂,液-液萃取法处理血浆样品。色谱柱:Phenomenex C18;流动相:0．05 mol·L-1磷酸二氢钠溶液(pH 3．2)-乙腈(90:10)。检测波长:λex =230 nm,λem=310 nm。流速:1．0 ml·min-1。结果:线性范围4．6-883．2 ng·ml-1;最低检测浓度为2．3 ng·ml-1;绝对回收率为95．1％;日内RSD小于3．9％,日间RSD小于5．5％。结论:方法灵敏准确,简便可行,适于阿替洛尔的治疗药物浓度监测。Objective ： To establish a RP-HPLC/fluorimetric detection method for content determination of atenolol in human plasma. Method： The extraction solvent was ethylacetate - isopropanol （8：1 ）. Liquid - liquid extraction was used to treat with human plasma. A Phenomenex C18 column was used. The mobile phase was 0.05 mol·L^-1 phosphate buffer （ pH 3.2） -acetonitrile （90： 10）. The detective wavelength was as follows： λex =230 nm,λem =310 nm. The flow rate was 1.0 ml·ml^-1. Result： There was a good linear relationship within the range of 4.60-883.2 ng·ml^-1 , and the detective limit of concentration was 2.3 ng·ml^-1. The absolute recovery was 95.1%. The inter-day RSD was less than 3.9% and intra-day RSD was less than 5.5%. Conclusion： This method is sensitive, accurate and simple. It is suitable for monitoring therapeutic concentration of atenolol.

关 键 词：阿替洛尔 血药浓度 反相高效液相色谱法 

分 类 号：R97[医药卫生—药品]