大鼠胚胎皮肤淋巴管血管内皮生长因子受体-3的表达  

EXPRESSION OF VEGFR-3 IN LYMPHATIC ENDOTHELIUM OF THE SKIN IN RAT EMBRYOS

作　　者：马晶[1] 赵玲辉[1] 刘宝全[1] 张雅芳[1] 杨树才[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,哈尔滨150086

出　　处：《解剖学报》2005年第5期528-531,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30270697)

摘　　要：目的观察血管内皮生长因子受体-3(VEGFR-3)蛋白和mRNA在大鼠胚胎发育不同时期皮肤淋巴管内皮的表达模式,明确VEGFR-3在胚胎淋巴管发生中的生物功能。方法应用免疫组织化学技术(SABC法)和原位杂交技术对61例大鼠胚胎第15 d和第21 d皮肤组织进行VEGFR-3蛋白和mRNA检测。结果皮肤淋巴管内皮细胞阳性表达VEGFR-3蛋白和mRNA,在大鼠胚胎发育的第15 d和第21 d,VEGFR-3蛋白在淋巴管内皮的阳性表达率分别是38.71%(12/31)和73.33%(22/30),第21 d胚胎皮肤淋巴管内皮VEGFR-3蛋白的表达水平明显高于第15d胚胎淋巴管内皮VEGFR-3蛋白的表达水平(χ2=7.408P<0.01)。VEGFR-3 mRNA在淋巴管内皮的阳性表达率分别是61.29%(19/31)和80.0%(24/30)。结论1.大鼠胚胎皮肤淋巴管阳性表达VEGFR-3蛋白和VEGFR-3mRNA。2.随着大鼠胚胎的发育,VEGFR-3蛋白和mRNA在皮肤淋巴管的阳性表达均表现为上升的趋势。3.VEGFR-3在大鼠胚胎皮肤淋巴管的发育中起一定的调节作用。Objective To analyse the expression patterns of the vascular endothelial growth factor receptor 3 （VEGFR-3） at the protein and mRNA level, and to detect the biological function of VEGFR-3 in the lymphanglogenesis of the embryos. Methods A total of 61 specimens of skin were investigated by immunohistochemical staining with a polyclonal antibody against VEGFR-3 in embryo 15-day-old（El5） and in embryo 21-day-old（E21）. The expression of VEGFR-3 mRNA was studied in situ hybridization. Results The positive expression of VEGFR-3 can be seen in lymphatic vessels of the embryonic skin. The occurrences of VEGFR-3 protein in lymphatic vessels in El5 and in E21 were 38.71% （12/31） and 73.33% （22/30） respectively, the expression level of VEGFR-3 protein in E21 was significantly higher than that in El5（ χ^2 = 7.408, P 〈 0.01）. The positive expression rates of VEGFR-3 mRNA in lymphatic vessels of the embryo skin were 61.29% （19/31） and 80.0% （24/ 30） separately. Conclusion 1.VEGFR-3 protein and mRNA can be detected in lymphatic vessels of the rat embryonic skin. 2.Along with the development of embryos, the expression of VEGFR-3 protein and mRNA in lymphatic vessels have an increasing tendency. 3.VEGFR-3 plays a major role in lymphangiogenesis in rat embryonic skin.

关 键 词：血管内皮生长因子受体-3 淋巴管 免疫组织化学 皮肤 胚胎 

分 类 号：R322.2[医药卫生—人体解剖和组织胚胎学]