低切应力对动脉粥样硬化血管平滑肌细胞Bcl-2和Bax蛋白表达的影响  

EFFECTS OF LOW SHEAR STRESS ON BCL-2 AND BAX EXPRESSION OF VASCULAR SMOOTH MUSCLE CELLS IN COMMON CAROTID ATHEROSCLEROSIS

作　　者：王溪涛[1] 刘波[2] 张炎[1] 姜宗来[2] 

机构地区：[1]上海第二军医大学解剖学教研室,上海200433 [2]上海交通大学医学院力学生物学与医学工程实验室,上海200240

出　　处：《解剖学报》2005年第5期567-569,共3页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(10132020)

摘　　要：目的研究低切应力对颈总动脉粥样硬化形成及其血管平滑肌细胞凋亡的影响。方法应用低切应力颈总动脉粥样硬化模型,采用免疫组织化学方法观察大白兔血管平滑肌细胞的Bcl-2及Bax蛋白表达的变化。结果颈总动脉血管平滑肌细胞(VSMC)的Bcl-2表达减少、Bax表达增高。结论在低切应力作用下动脉粥样硬化的形成过程中,低切应力与高脂协同作用,可使促进凋亡的因素增强,抑制凋亡的因素减弱,从而调控VSMC的凋亡。Objective To investigate effects on low shear stress in atherosclerosis formation and apoptosis of vascular smooth muscle celIs（VSMC） of common carotid artery. Methods An atherosclerosis model of common carotid artery under low shear stress has been set up, and expression of Bcl-2 and Bax protein in rabbit＇ s VSMC were studied by immunohistochemical method. Results The expressions of Bcl-2 protein decreased while Bax protein increased under low shear stress in atherosclerosis formation. Conclusion The results suggest that the low shear stress may regulate VSMC through the changes of apoptosis- associated proteins.

关 键 词：低切应力 颈总动脉 粥样硬化 血管平滑肌细胞 细胞凋亡 免疫组织化学 大白兔 

分 类 号：R543.502[医药卫生—心血管疾病]