乙肝病毒前S_1蛋白检测在诊断乙型肝炎病毒复制中的意义  

Significance of detecting Pre-S_1 protein in diagnosing viral replication in patients with hepatitis B

作　　者：黄伟刚[1] 陈青龙[2] 张慧萍[1] 林津 

机构地区：[1]广东省江门市人民医院检验科,529051 [2]广东省中山大学达安基因诊断中心

出　　处：《临床医学》2005年第10期5-7,共3页Clinical Medicine

摘　　要：目的探讨前S1蛋白(Pre-S1蛋白)的检测在诊断乙型肝炎病毒复制中的意义。方法采用酶联免疫吸附试验(ELISA)法检测138例携带不同乙型肝炎病毒标志物(HBV-M)和乙肝患者血清Pre-S1蛋白;采用荧光定量PCR(FQ-PCR)法检测HBV-DNA,并对结果进行分析。结果HBsAg、HBeAg、抗HBcAg均阳性者,Pre-S1蛋白和HBV-DNA检出率分别为90.4%和95.2%,两者间差异不显著(P>0.05),该组患者Pre-S1蛋白、HBV-DNA与HBeAg的检出符合率较高。HBeAg阴性患者HBV-DNA与Pre-S1蛋白仍有不同检出率,均明显低于HBeAg阳性患者(P<0.01),显示部分HBeAg阴性患者仍存在病毒复制。结论Pre-S1蛋白能敏感地反映乙型肝炎病毒复制的情况,尤其可反映HBeAg阴性的乙肝患者病毒复制。Objective To study the clinical significance of detecting HBV Pre - S1 protein in diagnosing viral replication in patients with hepatitis B. Methods 138 patients with hepatitis B were included in the study. HBV Pre - S2 protein and HBV - M were detected by ELISA, HBV - DNA was detected by fluorescent quantification PCR. Results The positive rates of Pre - S1 protein and HBV - DNA in patients with HBsAg, HBeAg, anti - HBcAg（ ＋ ） were 90.4% and 95.2% respectively. The positive rates of Pre - S1 protein and HBV - DNA in patients with HBeAg（ - ） were much lower than the HBeAg（ ＋ ） group,and it represented some patients with HBeAg（ - ） still had viral replications. Conclusion Compared with HBeAg,HBV Pre- S1 protein can reflect the situation of viral replication more sensitively in patieuts with hepatitis B.

关 键 词：前S1蛋白 乙型肝炎病毒 ELISA法 荧光定量PCR 乙型肝炎病毒复制 蛋白检测 乙肝病毒前S1 酶联免疫吸附试验(ELISA) PRE-S1蛋白 HBeAg阴性 HBV-DNA 诊断 乙型肝炎病毒标志物 

分 类 号：R446.6[医药卫生—诊断学]