ANA、ds-DNA抗体联合检测自身免疫病的临床应用研究  

Clinical application of co-detections of ANA, Anti-ds-DNA in autoimmune diseases

作　　者：谭立明[1] 吴越[2] 陈炜[3] 牟秀萍[1] 李华[1] 秦和昌[1] 王外梅[1] 

机构地区：[1]江西医学院第二附属医院,南昌330006 [2]南昌市疾病预防控制中心 [3]南昌大学职工医院

出　　处：《江西医学检验》2005年第5期408-410,共3页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的探讨ANA、ds-DNA抗体联合检测对自身免疫病的临床应用及意义。方法应用间接免疫荧光法检测ANA、ACA、ACYA和ds-DNA抗体,并进行回顾性分析。结果①245例各种自身免疫病患者检测ANA、ACA、ACYA、ds-DNA抗体结果,ANA在SLE患者中最高,阳性率为97.67%;ACA抗体在SSc患者中最高,阳性率为(80%);ACYA抗体检测在PM/DM患者中最高,阳性率为28.57%;ds-DNA抗体亦在SLE患者中最高,阳性率为62.79%;②180例ANA阳性结果分布情况为均质型以MCTD患者最多,阳性数为27例;斑点型和核周型则均以SLE患者为最多,有54例和12例阳性;核仁型则与SSc患者为最多,阳性3例;③13例ACYA抗体阳性结果以均质型为主,均质型阳性有9例,并且均是SLE患者。结论应用ANA、ACA、ACYA和ds-DNA抗体联合检测在自身免疫病患者诊断中具有互补性,并能提高阳性检出率。对自身免疫病的诊断分型、治疗预后均有重要的临床价值。Objective To investigate the value of co-determinations of ANA, anti-ds- DNA in autoimmune diseases（AID）. Method ANA, ANCA, ACYA and anti-ds-DNA were dectected by indirect immuno- fluorescence. Results ① In 245 AID cases of positive rate of ANA was 97.6% in SLE, with 80% of ACA in SSc, 28.57% of ACYA in PM/DM, 62.79% of an- ti-ds-DNA was in SLE respectively. ② In 180 positive cases of ANA, 27 cases were the type of homogeneous （most in MCTD）; 54 cases were the type of speckled and 12 case were the type of membranous（most in SLE）; 3 cases were the type of nucleolar（most in SSc）. ③There were 9 positive cases of type homogeneity in 13 ACYA positive cases. Conclusions In diagnosing AID, co-detections of ANA, ACA, ACYA and anti-ds-DNA which are complementary with each other, upgrade the detectable rate and have significat clinical value in diagnostic classification and prognosis of the diseases.

关 键 词：抗核抗体 抗双链DNA抗体 抗着丝点抗体 抗细胞浆抗体 自身免疫病 系统性红斑狼疮 

分 类 号：R446.62[医药卫生—诊断学] R392[医药卫生—临床医学]