稻瘟菌诱导的水稻WRKY基因OsWRKY52的分离和鉴定(英文)  

Isolation of a Rice WRKY Gene OsWRKY52, Whose Expression Is Induced by Magnaporthe grisea

作　　者：王海华[1,2] 谢科[1,2] 吴坤陆[1,2] 郭泽建[3] 

机构地区：[1]浙江大学农业与生物技术学院 [2]中国农业大学植物病理系,北京100094 [3]中国农业大学植物病理系

出　　处：《生物化学与生物物理进展》2005年第10期937-946,共10页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划(973)资助项目(G2000016203);国家自然科学基金资助项目(30370139,30471122)~~

摘　　要：WRKY蛋白参与植物对生物或非生物胁迫反应和一些发育、代谢过程,在植物中组成一个转录因子大家族.从水稻cDNA文库中分离到一个新的WRKY基因——OsWRKY52cDNA,包括一个1719bp的开放读码框,推测编码一个由572个氨基酸组成的蛋白质,与燕麦(Avenasativa)AsWRKY1具有54%的氨基酸一致性.该基因被非亲和性稻瘟菌快速诱导.凝胶阻滞实验结果表明,原核表达的OsWRKY52能与水稻PR1a启动子上的W盒元件特异结合.采用酵母单杂交体系的方法证明了OsWRKY52具有转录激活活性,其丝氨酸岛、苏氨酸岛和C端的富酸性氨基酸区是负责转录激活的区域.这些结果提示OsWRKY52作为一个转录激活子,可能参与植物对稻瘟菌的应答反应.WRKY proteins, a big family of transcription factors, are involved in regulation diverse developmental and physiological processes in plants. Here, a novel WRKY gene, OsWRKY52, was isolated from a rice cDNA library. This gene included an open reading frame of 1 719 bp in length, and the deduced polypeptide contained 572 amino acids,sharing 54% identity with a WRKY1 protein from Avena sativa. Expression of OsWRKY52 gene was induced rapidly by Magnaporthe grisea in the incompatible interaction with rice plant. OsWRKY52 protein, expressed prokaryotically bound specifically to W box cis elements derived from the promoter of a rice PR1a. Transcriptional activation assay was performed by a yeast one- hybrid method. Regions of transactivation were identified to be the N-terminal serine- and threonine-rich islands and the C-terminal acidic domain of OsWRKY52. These results suggest that OsWRKY52, as a transcription activator, may be involved in defense responses against Magnaporthe grisea in rice plants.

关 键 词：DNA结合 稻瘟菌(Magnaporthe grisea) 水稻(Oryza sativa) 转录激活 转录因子 WRKY 

分 类 号：Q5[生物学—生物化学] Q7