DNA指纹图技术分析微生物肥料菌种组成稳定性  

Bacterial composition of microbial fertilizers as evaluated by DNA fingerprinting techniques

作　　者：王凌华[1] 赵勇[2] 李武[1] 赵立平[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240 [2]南京农业大学生命学院微生物系,江苏南京210095

出　　处：《中国微生态学杂志》2005年第5期321-323,共3页Chinese Journal of Microecology

基　　金：863计划(2004AA214071);上海市科技兴农重点攻关项目[(2002)第4-4-2号]资助项目

摘　　要：目的分析2种市售微生物肥料在不同生产批次中微生物菌种组成的稳定性.方法利用ERIC-PCR基因组DNA指纹图技术和16S rDNA V3扩增一温度梯度凝胶电泳(PCR-TGGE)指纹图技术.结果这2种微生物肥料在同一个生产批次不同包装之间的2种DNA指纹图谱相似性较高,分别在78%～95%和96%～100%,表明同一个批次内的菌种组成比较一致.但其在不同的生产批次之间菌种组成差异存在显著性,反映在2种DNA指纹图谱上,不同生产批次样品间ERIC-PCR指纹图相似性最低只有10%,PCR-TGGE指纹图相似性最低为46%.结论通过ERIC-PCR和PCR-TGGE DNA指纹图技术可以对微生物肥料中菌种组成的稳定性进行快速、准确的分析,如何保持菌种组成在批次之间的稳定一致,是复合菌种微生物肥料质量控制中面临的难题.Objective To analyzed the-batch-to-batch stability of bacterial composition of two commercial microbial fertilizers （G and Y）. Method Evaluated by ERIC-PCR （Enterobacterial Repetitive ]ntergenic Consens- PCR） and PCR-TGGE （PCR-temperature gradient gel electrophoresis） of 16S rDNA V3 region. Results The similarity coefficient （Dice） of DNA fingerprints （ERIC-PCR and PCR-TGGE） of reduplicate samples in the same production batch was 78% - 95 % and 96% - 100% respectively. Among the different production batches, the lowest similarity coefficient was 10% in ERIC-PCR fingerprints and 46% in PCR-TGGE fingerprints. This showed that the stability of bacterial composition among samples in the same production batch was higher than those from different production batches. Condusions ERIC-PCR and PCR-TGGE fingerprinting had a good potential as a quality control technology to evaluate and monitor the stability of bacterial composition of microbial fertilizers. This work also indicates that how to maintain the stability of bacterial composition was an important problem in quality control of microbial fertilizer production.

关 键 词：微生物肥料 ERIC-PCR PCR-TGGE DNA指纹图 质量控制 

分 类 号：Q786[生物学—分子生物学]