牧草根际3种平脐蠕孢菌培养性状及对营养的利用能力  

The culture characteristic and nutrition utilization of three species of Bipolaris

作　　者：陈秀蓉[1] 南志标[2] 杨成德[1] 任继周[1] 李亚利[1] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2005年第5期99-104,共6页Acta Prataculturae Sinica

基　　金：国家重点基础发展规划项目(G2000018602);国家自然科学基金项目(30471232)资助

摘　　要：首次对我国典型草原牧草根际特异平脐蠕孢菌、高粱平脐蠕孢菌及麦根腐平脐蠕孢菌3种真菌的培养特性进行了研究.结果表明,3种平脐蠕孢菌菌丝生长的温限均为10～35℃,最适温度30℃.在试验的11种碳源中,D-果糖等碳源对3种平脐蠕孢菌菌丝生长有明显的促进作用,菊糖、氯醛糖有较强的抑制作用.在试验的12种氮源中,亮氨酸、L-谷氨酸、硝酸铵有明显的促进作用,而L-精氨酸、尿素、碳酸铵、甘氨酸有较强的抑制作用.高粱平脐蠕孢菌对多种碳、氮源利用能力较强,麦根腐平脐蠕孢菌则较弱.3种平脐蠕孢菌在不同碳、氮源培养基上菌落颜色、大小、边缘整齐度有明显差异.This study focused on the characteristics of Bipolaris specifera , B. sorghicola and B. sorokiniana when grown in a culture. These species are from the grass rhizome of the steppe in eastern Gansu. The results showed hyphae from all three species could grow in the temperature range of 10-35℃, with the optimum hyphae growth occurring at 30℃. The hyphae grew fast on media with D-fructose as the C-source and either leucine, L-glutamic acid or ammonium nitrate as the N-source, but grew slowly on media with inulin or chloralose puriss as the C-source and either L-arginine, urea, ammonium carbonate or glycine as the N-source. B.sorghicola＇s ability of to utilize the C-source and N-sources was the best of the three species, with B. soroteiniaria the worst. There were evident differences in color, size and edge of clony between the Bipolaris species.

关 键 词：平脐蠕孢菌 培养特性 碳源 氮源 

分 类 号：S54[农业科学—作物学]