中药治疗激素性骨坏死的蛋白质组学分析  

Bone Proteomic Analysis About Chinese Medicine Action on Rat Glucocorticoid-induced Model of Osteonecrsis.

作　　者：刘建仁[1] 樊粤光[1] 王海彬[1] 史风雷[1] 熊莉华[1] 何伟[1] 彭宣宪[2] 王三英[2] 

机构地区：[1]广州中医药大学附一属院,广州510405 [2]厦门大学生命科学学院

出　　处：《中国中医骨伤科杂志》2005年第5期4-10,共7页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：获得国家自然科学基金资助(30273631;3010244);广东省自然科学基金(020778);广东省教育厅基金(Z02011)

摘　　要：目的:用蛋白质组学的研究方法进行激素性骨坏死的病理和中药作用机理的研究,有利于临床治激素性骨坏死。方法:按照常用激素性骨坏死的造模方法,建立大鼠坏死模型,并设立中药治疗组及空白对照组,经过6周处理后经骨形态学检查,确定骨坏死造模成功,处死动物取股骨和肱骨,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,找到各组间差异蛋白质点,进一步行胶内酶切,M AD ITOF/M S质谱分析,得到各差异点的蛋白质肽指纹图谱,结合蛋白质生物信息库(M atrix sc ience L td database),对各蛋白质进行初步鉴定。结论:初步鉴定了3个差异蛋白,分别为阻凝蛋白重链ⅡB、磷脂谷胱甘肽过氧分酶及泛素化酶E 2(MW:17 kd),初步认为这三种蛋白质在激素性骨坏死的发病及中药治疗过程中发挥着重要调控作用。Objective：To study the mechanism about glucocorticoid-induced osteonecrosis and Chinese Medicine action on osteonecrosis in proteomics ,it is beneficial to prevention and cure this disease. Methods：Rat was randomly divided into three groups：cortrol group,osteonecrosis model group and Chinese Medicine therapy group. In the present proteomic study ,we characterized the potential effects of glucocorticoids and Chinese Medicine on protein expression in rat bone. Using two dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins. Conclusion：Three proteins were identified as proteins similar to Myosin heavy chain I B,Phospholipid hydroperoxide glutathione peroxidase and ubiquitin - conjugating enzyme E2-17kD. These proteins have been documented to be glucocorticoid-rehed proteins. These results can provide valuable experimental evidences for the research of the molecular mechanism of osteoneerosis response to glueocorticoids and Chinese Medicine in bone.

关 键 词：双向电泳 肽质量指纹谱 基质辅助激光解吸/飞行时间质谱 骨坏死 激素 蛋白质组学 中药 激素性骨坏死 蛋白质组学 中药治疗 学分 中药作用机理 造模方法 形态学检查 蛋白质分子 结合蛋白质 

分 类 号：R681[医药卫生—骨科学] R730.43[医药卫生—外科学]