土耳其斯坦东毕吸虫肌动蛋白基因的克隆及其序列分析  

CLONING AND SEQUENCE ANALYSIS OF ACTIN GENE OF ORIENTOBILHARZIA TURKESTANICUM

作　　者：魏佳[1] 何国声[2] 徐梅倩[2] 姚宝安[1] 

机构地区：[1]华中农业大学,武汉430070 [2]中国农科院上海家畜寄生虫病研究所,上海200232

出　　处：《中国兽医寄生虫病》2005年第4期1-8,共8页Chinese Journal of Veterinary Parasitology

摘　　要：目的利用RTPCR和RACE技术克隆土耳其斯坦东毕吸虫肌动蛋白基因全长序列并进行序列分析。方法根据日本血吸虫和曼氏血吸虫肌动蛋白基因的保守区设计引物,利用RTPCR扩增出土耳其斯坦东毕吸虫的肌动蛋白基因的大片段,再结合RACE技术分别得到肌动蛋白的3’端和5’端,将三部分序列拼接后获得肌动蛋白基因的全长cDNA序列并进行序列分析。结果成功克隆了土耳其斯坦东毕吸虫肌动蛋白的全长cDNA并提交GenBank,登录号为DQ017265,核酸序列比对表明东毕吸虫肌动蛋白基因的核苷酸序列和日本血吸虫、曼氏血吸虫的同源性分别为90%和91%。结论土耳其斯坦东毕吸虫肌动蛋白的全长cDNA的克隆为进一步表达及其生物学性能的分析提供了理论基础。The partial coding region of Actin was cloned using RT-PCR. The 3 ＇ end fragment and 5 ＇ end fragment of Actin were obtained by 3＇ RACE and 5＇ RACE . The full-length cDNA sequence was gotten by splicing sequences of the three fragments. The cDNA sequence has been submitted to GeneBank and its accession number is DQ017265. The identity of Actin of Orientobilharzia turkestanicum is 90% with Schistosoma japonicum at the nuclei acid level, and the counterpoint is 91% with Schistosoma mansoni. The cloning of Actin lay a the theoretical foundation for the further expression and biological analysis of Actin full-length cDNA sequence.

关 键 词：土耳其斯坦东毕吸虫 肌动蛋白基因 克隆 序列分析 

分 类 号：S858.9[农业科学—临床兽医学]