运动性动情周期抑制雌性大鼠的骨结构与骨组织OPG mRNA、OPGL mRNA的表达  

The Structure and Level of OPG Mrna,OPGL mRNA of Bone in Exercise-induced Estrous Cycle Inhibited Female Rats

作　　者：高丽[1] 郑陆[1] 李晓霞[1] 田中[2] 隋波[1] 李晔[1] 万利[1] 周瑞霞[3] 

机构地区：[1]山东体育学院基础理论系,济南250063 [2]山东体育学院科研处,济南250063 [3]山东体育学院运动系,济南250063

出　　处：《天津体育学院学报》2005年第6期22-25,共4页Journal of Tianjin University of Sport

基　　金：山东省教育厅科技计划项目(项目编号:J04E11)

摘　　要：研究2月龄雌性SD大鼠进行10周大强度跑台运动后出现的运动性动情周期抑制现象,以模拟长期耐力运动训练后女运动员出现的运动性闭经病理现象,观察运动性动情周期抑制对骨组织结构的影响,探讨OPG、OPGL在运动性闭经致骨质降低中的作用。运用光镜、透射电镜观察股骨、椎骨的显微、超微结构、RT-PCR法定量测定大鼠股骨组织的OPG mRNA、OPGL mRNA的表达。结果与对照组相比,运动性动情周期抑制雌性大鼠的显微结构显示皮质骨变薄,破骨细胞的数目增多,松质骨的骨小梁数目减少、纤细、排列稀疏,超微结构显示衰老骨细胞的数目增多,少数细胞呈现凋亡早期特征,同时骨组织OPG mRNA表达降低,OPGL mRNA表达增加。结论:10周大强度跑台运动致运动性动情周期抑制雌性大鼠骨结构的完整性破坏,骨组织OPG/OPGL表达的降低是影响运动性动情周期、抑制雌性大鼠骨结构变化的重要原因之一。Objective： 2-month female SD rats carried through high intensity training in treadmill for 10 weeks. After training, rats displayed that their estrous cycle were inhibited to simulate phenomena of athlete secondary amenorrhea. We observed the change of bone＇s structure in exercise-induced estrous cycle inhibited female rats. And we studied the function of OPG and OPGL in athletic Secondary Amenorrhea. Method： We used microscopes and transmission electron microscopes to observe the structure of femur and vertebra in rats. Then we used RT-PCR to assay the level of OPG mRNA.OPGL mRNA in bone. Result： The microscopes result showed context were thinner and the number of osteoclast were increased and the number of girder were fewer and thinner in rats with estrous cycle inhibited. The transmission electron microscopes result showed the number of old bone cell and apoptosis cell were more than that in control. The level of OPG mRNA was elevated and the level of OPGL mRNA was decreased in bone of exercise-induced estrous cycle inhibited female rats than that in bone of control. Conclusion： All results showed high intensity training affected the integration of bone＇structure in exercise-induced estrous cycle inhibited female rats. The results indicated the decreased expression level of OPG/OPGL was the importance ingredient on the change of bone＇s structure in exercise-induced estrous cycle inhibited rats.

关 键 词：运动性闭经 运动性动情周期抑制 雌性大鼠 骨 结构 OPG OPGL 

分 类 号：G804.5[文化科学—运动人体科学]