HPLC法测定白芍总苷胶囊中芍药内酯苷、芍药苷和苯甲酰芍药苷  

HPLC Determination of albiflorin, paeoniflorin, and benzoylpaeoniflorinin Total Glucoside of Paeony Capsule

作　　者：王巧[1] 刘荣霞[1] 毕开顺[2] 果德安[1] 

机构地区：[1]北京大学中医药现代研究中心,北京100083 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中草药》2005年第11期1630-1632,共3页Chinese Traditional and Herbal Drugs

基　　金：国家科技部资助项目(2002BA906A29);国家中医药管理局资助项目(2004ZX01)

摘　　要：目的建立HPLC对白芍总苷胶囊中芍药内酯苷、芍药苷和苯甲酰芍药苷同时定量的分析方法。方法采用HPLC法。色谱柱Zorbax SB-C18色谱柱(250mm×4.6mm,5μm);流动相A为乙腈,B为0.015%磷酸溶液,梯度洗脱程序为0min(8%A),5min(12%A),20min(20%A),25min(20%A);35min(45%A),40min(45%A);检测波长230nm;柱温30℃。结果芍药内酯苷、芍药苷和苯甲酰芍药苷质量浓度与峰面积均呈良好的线性关系。平均回收率芍药内酯苷为96.99%,RSD为0.56%;芍药苷为103.6%,RSD为0.77%;苯甲酰芍药苷为104.2%,RSD为1.35%(n=5)。结论该方法分离度好,简便易行,具有良好的重现性,可用于白芍总苷胶囊中芍药内酯苷、芍药苷和苯甲酰芍药苷的同时测定。Objective An HPLC method was developed for the determination of albiflorin, paeoniflorin, and benzoylpaeoniflorin in Total Glucoside of Paeony Capsule （TGPC）. Methods HPt.C analysis was performed on a Zorbax SB-C18 column （250 mm× 4.6 mm, 5 μm） with mixture of acetonitrile （A） and 0. 015% phosphoric acid solution as mobile phase in gradient mode. The concentrations of solvent A were 8%, 12%, 20%, 20%, 45%, and 45% at 0, 5, 20, 25, 35, and 40 min, respectively; the detection wavelength was 230 nm and the column temperature was 30 ℃. Results The standard curves of albiflorin, paeoniflorin, and benzoylpaeoniflorin showed a good linearity and the average recoveries were 96.99% with RSD 0. 56% for albiflorin, 103.6% with RSD 0.77% for paeoniflorin, and 104.2% with RSD 1.35% for benzoylpaeoniflorin （n=5）, respectively. Conclusion The method is quick, simple, and repeatable for the determination of albiflorin, paeoniflorin, and benzoylpaeoniflorin in TGPC simultaneously.

关 键 词：白芍总苷胶囊 芍药内酯苷 芍药苷 苯甲酰芍药苷 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]