淫羊藿提取物对去卵巢大鼠骨转换率增强的抑制作用  

Inhibitory effect of Herba Epimedii extract on bone turn-over of ovariectomized rats

作　　者：解芳[1] 张岩[1] 吴春福[1] 姚新生[2] 黄文秀[3] 

机构地区：[1]沈阳药科大学药学院药理系,辽宁沈阳110016 [2]沈阳药科大学中药学院天然药物化学系,辽宁沈阳110016 [3]国家中药与分子药理重点实验室

出　　处：《中草药》2005年第11期1667-1670,共4页Chinese Traditional and Herbal Drugs

基　　金：香港特别行政区大学教育资助委员会资助(AOE/P-10/01)

摘　　要：目的研究淫羊藿提取物对去卵巢大鼠骨转换的影响。方法取3月龄SD大鼠手术切除双侧卵巢,1个月后ig给予阳性药17β-雌二醇(2mg/kg)以及淫羊藿提取物混悬液(110mg/kg),连续给药3个月。采用全自动生化分析仪测定血清中钙(s-Ca)、磷(s-P)的量和碱性磷酸酶(ALP)的活性及尿中钙(u-Ca)、磷(u-P)和肌酐(Cr)的量。利用ELISA试剂盒测定尿脱氧吡啶酚(DPD)的量。取胫骨近端制成不脱钙骨切片进行骨组织形态计量学分析。结果与假手术组相比,去卵巢大鼠s-Ca、s-P没有变化,ALP活性增强,u-Ca排出显著升高,尿中DPD与Cr的比值(DPD/Cr)显著增加,子宫萎缩。骨小梁面积百分比降低,骨小梁间隙增加。ig给予雌二醇或淫羊藿提取物3个月,血清ALP活性降低,u-Ca排出量减少,尿中DPD/Cr降低,骨小梁增加,且骨小梁间隙减小。雌二醇组大鼠子宫增生,淫羊藿组大鼠子宫与模型组相比没有变化。结论淫羊藿提取物能够显著抑制由于雌激素缺乏引发的骨转换增强,调整骨形成和骨吸收的关系,对遏制去卵巢造成的骨质疏松有一定的作用,且长期服用对子宫没有刺激作用。Objective To investigate the effect of Herba Epimedii extract on bone turn-over in ovariectomized rats. Methods Three-month old female SD rats were ovariectomized and Sham-operated respectively. One month after ovariectomy （OVX）, the rats were given Herba Epimedii extract （110 mg/ kg） and 17β-estrogen （2 mg/kg） by ig for three months continuously. Serum contents of Ca （s-Ca）, P （s- P）, and ALP as well as urine contents of Ca （u-Ca）, P （u-P）, and creatinine （Cr） were detected. Urine DPD/Cr was analyzed by ELISA kit. The proximal tibia was processed undecalcified sections of 5 μm for histomorphometric analysis. Results There was no change among all groups in the levels of s-Ca.s-P, and u-P. Serum ALP activity significantly increased in OVX rats with metratrophia compared with Sham group, as well as u-Ca and DPD/Cr. OVX group had lower trabecular bone mass with decreased area percentage of trabecular bone mass and increased suturae of trabecular bone mass. Both Herba Epimedii extract and 17β-estrogen treatment could make serum ALP activity inhibit, u-Ca and DPD/Cr reduce, trabecular bone mass increase, and suturae of trabecular bone mass constrict in tibia widen. Hysterauxesis was found in 17β-estrogen group, while no change was observed in Herba Epimedii extract group compared with model group. Conclusion Herba Epimedii extract could inhibit the high rate of bone turnover induced by OVX in rats, without substantial effects on the uterus.

关 键 词：淫羊藿 骨质疏松 卵巢切除术 

分 类 号：R285.5[医药卫生—中药学]