高效液相色谱法同时测定复方左旋氧氟沙星喷雾剂中2组分的含量  

Simultaneous Determination of 2 Components in Compound Levofloxacin Spray by HPLC

作　　者：黄良永[1] 杜士明[1] 张先洲[2] 

机构地区：[1]湖北省十堰市太和医院药学部,十堰市442000 [2]武汉大学人民医院,武汉市430060

出　　处：《中国药房》2005年第21期1659-1660,共2页China Pharmacy

摘　　要：目的:建立以高效液相色谱法同时测定复方左旋氧氟沙星喷雾剂中左旋氧氟沙星和更昔洛韦含量的方法。方法:色谱柱为 Dikma C18,流动相为0．025mol／L磷酸溶液-乙腈(87:13),检测波长为278nm,流速为1ml／min,柱温为30℃。结果:左旋氧氟沙 星、更昔洛韦检测浓度分别在12．5-400μg／ml(r=0．9 999)、6．25-200μg／ml(r=1．0 000)范围内线性关系良好,平均回收率 分别为99．90％(RSD=0．02％)、99．96％(RSD=0．06％)。结果:本法简便、快速、重现性好、回收率高,可同时测定复方左旋氧氟 沙星喷雾剂中2组分的含量,并可用于其质量控制。OBJECTIVE： To establish a HPLC method for the simultaneous assaying of levofloxacin and ganciclovir in compound levofloxacin spray.METHODS： The chromatographic column was Dikma C18 with column temperature at 30℃ ; the mobile phase was composed of 0.025mol/L H3O4 solutionacetonitrile（87 ： 13） with detection wavelength at 278nm and flow speed at 1ml/min.RESULTS：Good linear relations were achieved when the concentration ranges of levofloxacin and ganciclovir were 12.5-400μg/ml（ r = 0.9 999） and 6.25-200μg/ml（ r = 1.0 000） respectively, the recovery rates were 99.90% （RSD = 0.02% ） and 99.96% （RSD = 0.06% ） respectively. CONCLUSION：This method is convenient, fast, reproducible and with high recovery rates, which can be used to determine 2 constituents simultaneously of compound levofloxacin spray and control its quality.

关 键 词：高效液相色谱法 复方左旋氧氟沙星喷雾剂 左旋氧氟沙星 更昔洛韦 含量 

分 类 号：R927.2[医药卫生—药学]