莱克多巴胺单克隆抗体的制备与初步鉴定  

PREPARATION AND IDENTIFICATIONN OF MONOCLONAL ANTIBODIES AGAINST RACTOPAMINE

作　　者：曲勍[1] 齐孟文[1] 

机构地区：[1]中国农业大学核技术农业应用实验室,北京100094

出　　处：《核农学报》2005年第5期393-396,共4页Journal of Nuclear Agricultural Sciences

摘　　要：为了对莱克多巴胺进行酶联免疫法测定,经由混合酸酐法合成莱克多巴胺免疫原,免疫BALB/c小鼠,通过杂交瘤单克隆化技术最终得到了1株名为3E1-C9-E10的能稳定分泌抗莱克多巴胺的单克隆抗体的杂交瘤细胞株。经ELISA测定,细胞培养上清的效价为1×105,腹水的效价达1×107以上,与多巴酚丁胺有24%的交叉反应率,与沙丁胺醇、克伦特罗没有交叉反应率。In order to prepare the monoclonal antibody agaist Ractopamine （RCT）, RCT-conjugated antigen was produced by methods of mixed acid anhydride. The spleen cells of BALB/c mice immunized by RCT-BSA were fused with SP2/0 plasmacytoma cells using PEG4000. A hybridoma cell line of 3E1-C9-E10 was screened for specificity to RCT and cloned by limited dilution method , which secreted stable monoclonal antibodies against RCT with indirect ELISA titers of 1 × 10^5in supernatant , 1 × 10^7in astices. The McAb of 3E1-C9-E10 generally had 24% cross- reactivity to Dobutamine, and showed little or no cross - reactivity to Salbutamol and Clenbuterol.

关 键 词：莱克多巴胺 β-肾上腺素激动剂 单克隆抗体 BALB/C小鼠 杂交瘤细胞株 酶联免疫法 鉴定 制备 混合酸酐法 ELISA 

分 类 号：S852.43[农业科学—基础兽医学]