Galectin-3和CD44v6在甲状腺结节中的表达及其意义  

Significance of galectin-3 and CD44v6 expression in differential diagnosis of thyroid nodules

作　　者：王丽华[1] 赵咏桔[1] 王晓颖[2] 袁平[2] 许伟庆[3] 肖家诚[2] 徐锦屏[4] 罗邦尧[1] 

机构地区：[1]上海第二医科大学附属瑞金医院内分泌科 [2]上海第二医科大学附属瑞金医院病理科,200025 [3]上海第二医科大学病理教研室 [4]台州市中心医院病理科

出　　处：《中华肿瘤杂志》2005年第9期547-550,共4页Chinese Journal of Oncology

摘　　要：目的了解Galectin-3和CD44v6在甲状腺良恶性结节中的表达差异,探讨两项指标在鉴别甲状腺结节良恶性方面的临床应用价值。方法采用免疫组化ABC法,检测143例甲状腺良恶性结节的石蜡标本中Galectin-3蛋白及CD44v6蛋白的表达差异。结果Galectin-3和CD44v6在甲状腺癌中的阳性率显著高于良性结节及正常组织中的阳性率(P<0.01)。Galectin-3联合CD44v6检测在鉴别甲状腺结节良恶性方面的敏感性、特异性和诊断准确率分别为80.8%、93.4%和88.8%,而单独检测Galectin-3时,敏感性、特异性和诊断准确率分别为96.2%、90.1%和92.3%。结论Galectin-3和CD44v6(尤其是前者)的免疫组化检测有助于甲状腺癌的鉴别诊断。Objective To investigate the difference of galectin-3 and CD44v6 expression between benign and malignant thyroid nodules, and to envaluate their clinical value in distinguishing thyroid cancer from benign thyroid nodules. Methods The expression of galectin-3 and CD44v6 was immunohistochemically detected by the ABC method in 143 benign and malignant thyroid nodule samples. Results Expression of these two markers in benign thyroid nodules ： galeetin-3 was negative in 10 cases of para-eaneer normal tissue and 14 cases of benign nodules found in the other benign thyroid disease. It was weakly positive in 4 of 52 nodular goiter （ 7.7% ）. Also it was weakly positive in 2 of 22 follicular adenomas （9.1%）. But all three eosinophilie follicular adenomas were diffusely or focally positive for galeetin-3. CD44v6 was negative in 10 cases of para-eaneer normal tissue, but positive in 4 of 14 nodular lesions found in benign thyroid diseases （28.6%）. It was also positive in 16 of 52 nodular goiters （30.8%）, and weakly positive in 7 of 22 follicular adenomas （31.8%）. The two markers in maignant lesions： galeetin-3 was positive in 50 of 52 throid adenoeareinoma （96.2%）, CD44v6 was positive in 42 of 52 thyroid adenoeareinoma （80.8%）. The positive rate of galeetin-3 and CD44v6 expression in thyroid cancer was significantly higher than that in benign thyroid nodule and normal tissue（ P 〈0. 001 ）. The sensitivity, specificity and accuracy of galectin-3 combined with CD44v6 in differentiating benign from malignant thyroid nodule were 80. 8%, 93. 4%, 88.8% ; they were 96.2% ,90. 1% ,92.3% for Galectin-3 alone. Conclusion The immunohistochemical expression of galectin-3 and CD44v6 by the ABC method is significantly higher in thyroid cancers than in benign thyroid nodules, especially galectin-3 in thyrocyte being helpful in differentiating benign thyroid nodule from thyroid cancer.

关 键 词：GALECTIN-3 CD44V6 甲状腺肿瘤 CD44V6蛋白 甲状腺结节 甲状腺良恶性结节 免疫组化检测 其意义 免疫组化ABC法 诊断准确率 

分 类 号：R581[医药卫生—内分泌]