大鼠早期三级卵泡中的卵母细胞能在联合培养系统中成批适当成熟(英文)  

RAT OOCYTES IN EARLY TERTIARY FOLLICLES CAN ATTAIN APPROPRIATE MATURATION IN COMBINATION CULTURE SYSTEM

作　　者：颜桂军[1] 顾正[2] 孙兆贵[2] 左嘉客[2] 

机构地区：[1]复旦大学上海医学院,上海200032 [2]上海市计划生育科学研究所,上海200032

出　　处：《实验生物学报》2005年第5期432-440,共9页Acta Biologiae Experimentalis Sinica

基　　金：国家"973"项目(G1999055902)~~

摘　　要：我们建立了由离体同步未分化颗粒细胞、动情前期垂体前叶和Δ-4雄烯二酮组成的“联合培养系统”。该联合培养系统可模拟正常生理状态下各相关细胞间的协同作用,更有效地诱发经DES 刺激后所获得的大量大鼠早期三级卵泡中的卵母细胞同步达到适当成熟,从而获得符合正常生理标准的成熟卵球,使卵巢内大量卵母细胞得到充分利用。我们的实验表明,经DES刺激,每侧幼龄大鼠卵巢中平均可取得处在早期三级卵泡中的189枚卵母细胞。在联合培养系统中培养,78%的卵母细胞排出第一极体达到成熟。这些成熟的卵母细胞中88%可正常受精,93%卵裂,经体外培养96h, 有59%的受精卵可发育到桑椹或囊胚。2-细胞期胚胎移植后可发育为健康个体。因此,该联合培养系统为研究大鼠卵母细胞成熟、受精和早胚发育的分子机制提供了非常有效的实验模型。We have developed a “combination culture system”, composed of primary differentiated granulosa cells, proestrus anterior pituitary lobe and △-4-androstenedione. This cultured system effectively induce the maturation of oocytes from early tertiary follicles of diethylstilbestrol （DES）-treated rats. After DES stimulation, an average of 189 oocytes were obtained from early tertiary follicles of unilateral ovaries; 78% of these oocytes, when cultured in this culture system, extruded the first polar body, a criterion of oocyte maturation. Of the mature oocytes, the rate of normal fertilization and egg cleavage were 88% and 93%, respectively. After 96 h of culturing in vitro, 59% of the zygotes developed into morulae or blastocysts. Embryos implanted at the two-cell stage were able to develop into healthy individuals. Thus the combination culture system provides an experimental model in which the molecular mechanisms of egg maturation, fertilization, and embryonic development can be studied.

关 键 词：早期三级卵泡 卵母细胞 联合培养系统 适当成熟 卵母细胞成熟 培养系统 成熟卵 大鼠 卵泡 三级 

分 类 号：Q813.11[生物学—生物工程]