茉莉酸甲酯诱导下红豆杉细胞产生紫杉烷类物质群代谢轮廓分析  

Metabolic Profiling Analysis of Taxanes in Taxus chinensis Cell Cultures Elicited by Methyl Jasmonate

作　　者：赵春芳[1] 余龙江[1] 刘智[1] 栗茂腾[1] 周蓬蓬[1] 李丽琴[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《云南植物研究》2005年第5期557-564,共8页Acta Botanica Yunnanica

基　　金：863国家重点项目(102-12-06-01);2000年教育部高校骨干教师基金

摘　　要：红豆杉细胞培养物经甲醇提取和固相萃取粗分离,进行反相高效液相色谱分析,获得了约含13个与紫杉醇极性相关的化合物色谱图,通过质谱联用和对照品参照,归属了这些化合物组成。进一步利用茉莉酸甲酯(MJ)诱导细胞,比较这些组分在诱导前后的相对色谱峰面积变化,结果表明,所有紫杉烷组分的浓度在诱导后都提高,其中以taxchinin M及其结构类似物提高最显著,紫杉醇,B-Ⅲ和B-Ⅵ等比C14位取代的taxuyunnanine C及其衍生物的浓度增加幅度要大,提示MJ对紫杉醇合成中非有效乙酰化旁路代谢有促进作用,而对C14位取代物生成的旁路促进作用不明显。本文为紫杉醇的生物合成研究提供了新的思路和方法。Taxus chinensis cell cultures were extracted with methanol and then prepared by solid-phase extract prior to HPLC or LC-MS analysis. About 13 taxane compounds were identified or characterized by comparisons with LC-MS and standard referents. The profiling analysis of the taxanes by methyl jasmonate （MJ） elicitation indicated that the relative chromatographic peak areas of all these identified compounds were increased but the increasing rates differed. Taxchinin M and its structural analogue had the highest increment. The increasing rates of taxol, B-Ⅲ and B-Ⅵ were more than that of taxuyunnanine C and its derivatives, which were oxygenated at C-14. The results suggested that biosynthsis of taxol and its precursors were more sensitive than oxygenated taxanes at C-14 in response to methyl jasmonate ehcitations, while MJ may also elicit the formation of non-effective acetylated taxanes which bear acetate groups at C1,C2, C7, C9 and C13. The study may be interesting in metabolic profiling analysis for elucidating taxane biosynthesis.

关 键 词：紫杉烷 代谢轮廓分析 红豆杉细胞培养物 茉莉酸甲酯诱导 

分 类 号：S791.49[农业科学—林木遗传育种]