南瓜属植物RAPD-PCR反应体系的建立与应用  

Optimization and Application of RAPD-PCR System for Cucurbita

作　　者：庄东红[1] 黄秀丽[1] 吴奕瑞[1] 林奕韩 郑汉藩 

机构地区：[1]汕头大学生物系,广东汕头515063 [2]汕头市白沙蔬菜原种研究所,广东汕头515800

出　　处：《武汉植物学研究》2005年第5期422-426,共5页Journal of Wuhan Botanical Research

基　　金：广东省科技计划项目(2002A2070602)

摘　　要：采用混合DNA样品池,利用正交法对南瓜属植物RAPD反应条件进行了探讨,并利用建立起来的优化体系,从35条引物中筛选出7条引物,对供试中国南瓜、印度南瓜和黑籽南瓜3个栽培种的7个品种进行了RAPD分析,结果均获得了较多的多态性位点。RAPD-PCR反应扩增结果还显示,日本南瓜兼有中国南瓜和印度南瓜的特征带,聚类分析的结果则表明,它与中国南瓜的亲缘关系更近。Using DNA pooling,we established RAPD optimal conditions for Cucurbita through the orthogonal optimization method. With the founded system, 7 primers were selected form 35 primers to analyze 7 cultivars of C. moschata Duch, C. maxima Duch and C. ficifolia Bouche. The results showed that there were many polymorphic sites among the cultivars. The cultivar Riben Nangua had the distinct bands of C. moschata and C. maxima at the same time,and the results of cluster analysis showed it was near with C. moschata.

关 键 词：南瓜属 RAPD-PCR 混合DNA样品池 聚类分析 

分 类 号：Q949.782[生物学—植物学] Q346