血管内皮生长因子和转化生长因子β1在尖锐湿疣组织中的表达  

Expression of vascular endothelial growth factor and transforming growth factor beta 1 in condyloma acuminatum

作　　者：王俐[1] 邹金波[1] 黄向莹[1] 黄雄[1] 李佳玟[1] 

机构地区：[1]汕头大学医学院第一附属医院皮肤科,广东汕头515041

出　　处：《临床皮肤科杂志》2005年第11期729-731,共3页Journal of Clinical Dermatology

基　　金：广东省医学科研基金资助项目(A2003503)

摘　　要：目的:探讨血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)和转化生长因子β1(transforminggrowthfactorbeta1,TGFβ1)在尖锐湿疣(condylomaacuminatum,CA)组织中的表达及其可能作用。方法:观察并定量计数27例CA病变组织的血管数,应用逆转录(RT)-PCR和免疫组化方法检测VEGF和TGFβ1的表达情况,同时以17例正常包皮组织作为对照。结果:CA组织的血管数比正常包皮组织明显增多(t=5.059,P<0.01);VEGF和TGFβ1的mRNA在CA的表达水平分别为0.880±0.054、1.136±0.145;而二者在正常包皮组织内表达水平分别为0.448±0.095、0.784±0.085(P<0.05);VEGF和TGFβ1在CA组织除角质层外的表皮全层细胞均有强表达,正常包皮组织中为弱表达,且主要位于基底层细胞;VEGF和TGFβ1的表达呈显著正相关(r=0.792,P<0.001)。结论:CA组织细胞可产生并分泌较多的TGFβ1和VEGF,两者可能与CA组织中的血管生成有关。Objectives： To investigate the expression and effects of vascular endothelial growth factor（VEGF）and transforming growth factor beta I（TGFβ1）in patients with condyloma acuminatum （CA）. Methods： To observe and quantify the blood vessel density, and to detect expression of VEGF and TGFβ1 by RT-PCR and immunohistochemistry in 27 specimens of CA and 17 normal human foreskin tissues, respectively. Results： The blood vessel density in CA was significantly higher than that in normal foreskin tissue（t= 5.059, P〈0.01）. The expression of mRNA of VEGF and TGFβ1 was higher than that in the nor- mal foreskin （P〈0.05）. The distribution of VEGF and TGFβ1 in normal foreskin tissues was mainly in the basal layer,while in CA was throughout the epidermis except the stratum corneum, and the intensity of staining in CA was also significantly stronger than that in the normal foreskin. There was a striking positive correlation between the expression of VEGF and TGFβ1 in CA（r=0.792, P〈0.001）. Conclusions： TGFβ1 and VEGF could be produced and secreted by the involved cells of CA, which might be associated with the formation of new blood vessel in CA.

关 键 词：尖锐湿疣 血管内皮生长因子 转化生长因子Β1 

分 类 号：R752.53[医药卫生—皮肤病学与性病学] R392.12[医药卫生—临床医学]