电喷雾质谱法检测假单胞菌BS-03产鼠李糖脂及其自由酸前体(英文)  

Electrospray ionization mass spectrometric detection of rhamnolipids and their acid precursors in Pseudomonas sp. BS-03 cultures

作　　者：沈薇[1] 杨树林[1] 李校堃[1] 

机构地区：[1]南京理工大学生物工程研究所,南京210094

出　　处：《中国生物工程杂志》2005年第10期83-87,共5页China Biotechnology

基　　金：国家"十五"科技攻关资助项目(2001BA706B-18);南京市科技发展计划资助项目(200301045)~~

摘　　要：采用电喷雾质谱法可同时检测到假单胞菌BS-03发酵液提取物中的鼠李糖脂及其自由酸前体(3-羟基葵酸单体和二聚体)。根据ESI-MS/MS的双鼠李糖脂二级质谱图显示存在强度较高的m/z为205、247的特征碎片离子峰,而单鼠李糖脂中却不存在此特征碎片离子。同时自由酸前体的主要组分为C8,C10,C8C10/C10C8,C10C10。其同分异构体与鼠李糖脂同分异构体类似,其中短链位于羟基端的组分含量高于长链在羟基端的组分。A series of pseudomolecular and fragment ions attributed to 3-hydroxyalkanoic acids （HAs） and 3-（3-hydroxyalkanoyloxy） alkanoic acids （HAAs） were detected by ESI-MS among the rhamnolipids observed in Pseudomonas sp. BS-03 supernatant extracts. The structure of rhamnolipids, HAs and HAAs were deduced. According to ESI-MS/MS there were characteristic fragment ions at m/z 205, 247 in all dirhamnolipids while they did not exist in any monorhamnolipids. The most abundant free acid precursors were C8, C10, C8C10/C10C8 and C10 C10. Within an isomeric pair of HAAs, just like rhamnolipids the isomer with the shortest 3- hydroxyalkaloyl residues at the hydroxyl end was more abundant than the one with the heavier3-hydroxyalkaloyl acid at the same position.

关 键 词：电喷雾质谱法 检测方法 假单胞菌 BS-03 鼠李糖脂 自由酸前体 

分 类 号：Q939.112[生物学—微生物学]