LRP16基因抗紫外线DNA损伤作用的研究  

Protective effect of LRP16 over expression in HL-60 cell against DNA damage induced by ultraviolet

作　　者：卢学春[1] 朱宏丽[1] 楼方定[1] 韩卫东[1] 徐周敏[1] 于力[1] 

机构地区：[1]解放军总医院南楼血液科,北京100853

出　　处：《中华放射医学与防护杂志》2005年第5期477-479,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家973重点基金资助项目(2005CB522400);国家自然科学基金资助项目(39970318)

摘　　要：目的根据基因编码序列推测其氨基酸序列,预测LRP16基因编码蛋白的功能并通过实验加以证实。方法利用GeneBank数据库获取LRP16基因序列,然后推测出该基因编码蛋白质的一级结构,对该序列蛋白结构域的同源性进行搜索;将LRP16基因ORF插入pcDNA3.1,构建LRP16基因的真核表达质粒,采用Superfect稳定转染急性粒细胞白血病细胞系HL60,筛选稳定表达LRP16基因的细胞;以紫外灯照射筛选后有HL60稳定过表达的HL60细胞和对照细胞;采用单细胞凝胶电泳技术检测有LRP16过表达对HL60细胞增殖及DNA损伤后修复的影响。结果在LRP16基因理论蛋白序列的第148至315氨基酸残基之间具有与人类组蛋白H2A1C末端相类似的同源序列hismacro、COG2110和A1pp,同源性高达80%以上。LRP16基因的过表达具有抗紫外线引起HL60细胞DNA损伤和增强损伤后的修复作用。结论LRP16基因编码蛋白可能具有抗紫外线的DNA损伤作用。Objective LRP16 gene was first cloned by our study group. Based on its theory protein sequence with a H2A1C like domain, We predicted its product has protective effect against DNA damage induced by ultraviolet and validated the effect by experiment. Methods LRP16 gene sequence was obtained from GeneBank, then was its theory amino acid sequence. After that, we search possible domain on this assumed protein. At the same time, we constructed a pcDNA3.1-LRP16 vector which could express LRP16 gene stably in cell lines. Then the vector was transfected into HL-60 cell by Superfect and the cell which had stably LRP16 gene expression were left by G418 selected. HL-60 cells were caused DNA damage by ultraviolet. Single cell gel electrophoresis（SCGE） was used to test the DNA damage and repair pattern difference among a pcDNA3.1- LRP16, a pcDNA3.1 （ ＋ ） and HL30 group. Results There is a domain something like human hismacro, COG2110 and Alpp from amino acid residue 148 to 315. LRP16 over expression in HL-60 cell had the protective effect against DNA damage caused by ultraviolet. Conclusion LRP16 protein may have protective effect against DNA damage induced by ultraviolet.

关 键 词：LRP16基因 抗紫外线作用 DNA损伤 氨基酸序列 放射损伤 

分 类 号：R346[医药卫生—基础医学]