免疫印迹法分析抗SPIM抗体与临床应用  

Analysis on anti-SPIM antibodies by Western blotting and its clinical application

作　　者：黄邱朝[1] 商学军[1] 黄宇烽[1] 

机构地区：[1]南京军区南京总医院,解放军医学检验中心

出　　处：《实用男科杂志》1996年第2期91-94,共4页

摘　　要：采用聚丙烯酰胺凝胶电泳和免疫印迹法分析不育症病人抗精浆免疫抑制物抗体（ＳＰＩＭ－Ａｂ）对特异抗原的免疫反应，并与ＥＬＩＳＡ法进行比较。结果表明，免疫印迹法检测血清、清浆ＳＰＩＭ－Ａｂ阳性率分别为３０．３％（３２／１０７）和２９．０％（３６／１２４），ＥＬＩＳＡ法检测分别为３３．６％（３６／１０７）和３１．５％（３９／１２４），Ｘ２＝１２９．５８，Ｐ＜０．０１．免疫印迹法分析的６８份不育症病人的血清、精浆ＳＰＩＭ－Ａｂ阳性样本中，４９份（７２．１％）与特异抗原的分子量１１００００和（或）９４０００区带发生免疫反应，１９份（２７．９％）与分子量５２０００区带反应，１３份（１９．１％）与分子量３６０００区带反应。提示大分子量ＳＰＩＭ抗原可能更容易诱导高效价ＳＰＩＭ－Ａｂ产生，并形成抗原抗体免疫复合物干扰生育。The immune reactions of anti-seminal plasma immuno-inhibition material antibodies (anti-SPIM-Ab) with specific antigens in infertile patients were analyzed by SDS-PAGE and Western blotting, and the results were compared with those determined by ELISA. It was showed that the positive rates of anti-SPIM-Ab determined by Western blotting in sera and seminal plasma from infertile patients were 30. 0% (32/107) and 29. 0% (36/124), while 33. 6% (36/107) and 31. 5% (39/124) by ELISA respectively (X2=129. 58, P<0. 01). Among 68 samples with positive anti-SPIM-Ab in both sera and seminal plasma 49 (72. 1%) of samples reacted to the 110 kD and/or 94 kD bands of SPIM specific antigens, but 19(27. 9%) and 13 (19. 1%) of samples reacted with 52 kD and 36 kD band respectively. It is suggested that the SPIM antigens with high molecular weight may easily induce hith titer anti-SPIM antibodies, produce their immune complexes and interfere with fertility.

关 键 词：精浆免疫抑制物 抗体 免疫印迹 男性不育症 

分 类 号：R446.62[医药卫生—诊断学] R698.204[医药卫生—临床医学]