四氯化碳损伤小鼠肝脏基因表达谱的变化  

Changes of gene expression profiles in CCl_4 injured liver of mice

作　　者：陈勇[1] 程明[1] 夏启松[1] 杜鹏[1] 

机构地区：[1]湖北大学中药生物技术省重点实验室,湖北武汉430062

出　　处：《药学学报》2005年第10期898-902,共5页Acta Pharmaceutica Sinica

基　　金：国家重大基础研究前期专项(2002ccc00300);湖北省科技厅攻关项目(2003AA303B02).

摘　　要：目的研究CC l4肝损伤小鼠肝脏的基因表达谱,筛选与CC l4肝损伤相关的基因网络,探讨其肝损伤机制。方法提取小鼠的肝组织总RNA,经反转录分别用Cy3和Cy5荧光标记,制备用于芯片杂交的cDNA探针;cDNA探针与联合基因公司B ioStarM-141S小鼠基因表达谱芯片进行杂交,结果由扫描仪扫描并用软件进行分析统计。结果在CC l4肝损伤小鼠的基因表达谱中,有379条基因发生了差异性表达(2.69%)。其中,163条基因表达量明显上调,另外216条基因表达量明显下调。结论CC l4引起小鼠肝脏基因表达谱变化,利用小鼠基因表达谱芯片能大规模、高通量筛选与CC l4肝损伤相关基因,对进一步阐明CC l4及类似的化学肝毒物对肝脏的损伤机制有十分重要的意义。Aim To study the gene expression profiles between the CCl4 injured liver and normal liver in mice, and to screen the differentially expressed genes that relate to liver injury by CCl4 on a large scale using cDNA microarrays. Methods Male Kunming strain mice were divided into two groups： one was control group and another was CCl4 injured liver group that was given 0. 1% CCl4 oil solution ip at dose of l0 mL · kg^-1 every three days, totally for ten times. Then mRNA in livers of the two groups of mice was extracted, separately, and reversely transcribed to cDNA with the incorporation of different fluorescent-labeled dUTP as the hybridization probes. The mixed probes were hybridized to the cDNA microarrays. The fluorescent signal values were acquired by scanner and analyzed with statistical software. Results Among the 14 100 target genes, 379 genes were differentially expressed, in which 163 genes were up-regulated and the other 216 genes were down-regulated. They are closely related to a range of biological functions. Conclusion Using the cDNA microarray and experimental animal modeling technique, the differentially expressed genes of CCl4 injured liver in mice on a large scale could be studied. It is useful for further investigation of the injury mechanism of CCl4.

关 键 词：四氯化碳肝损伤 基因表达谱 基因芯片 

分 类 号：R349.31[医药卫生—基础医学]