东方铃蟾消化道5-羟色胺免疫活性细胞的免疫组织化学定位  

Immunohistochemical Localization of 5-HT Immunoreactive Cells in the Digestive Tract of Bombina orientalis

作　　者：李淑兰[1] 赵文艳[1] 冷超[1] 赵文阁[1] 

机构地区：[1]哈尔滨师范大学生命与环境科学学院,哈尔滨150080

出　　处：《动物学杂志》2005年第5期37-42,共6页Chinese Journal of Zoology

基　　金：黑龙江省科学技术计划项目资助(No.sy02-07)

摘　　要：应用5-HT抗血清,以ABC(avidin-biotin-peroxidase complex)免疫组织化学方法,对东方铃蟾(Bombinaorientalis)消化道内的5-HT免疫活性细胞(5-Hydroxytryptamine immunoreactive cell,5-HT cell)进行了免疫组织化学的定位研究和形态学观察,并探讨其分布型的成因及细胞形态与功能的关系。结果表明,5-HT细胞在东方铃蟾消化道的各个部位均有分布,其中以胃贲门部分布密度为最高:(55.73±10.67)个/mm2;胃幽门部次之:(42.99±5.25)个/mm2;直肠部最低:(6.37±4.78)个/mm2。5-HT细胞的形态多样:圆形、椭圆形、锥体形、长锥体形等,其中食管和直肠以圆形和椭圆形为主,小肠部则以长锥体形为主,其细长突起指向肠腔或固有膜。细胞分布于上皮基部、上皮细胞之间、腺泡上皮细胞之间或固有膜内;胃贲门部、胃体及胃幽门部5-HT细胞主要分布于腺泡上皮之间,也有的分布于胃上皮之间;肠部5-HT细胞主要分布于肠上皮之间。提示了东方铃蟾5-HT细胞分布型的形成与各部位消化功能及其食性和食物组成有关;5-HT细胞形态与其内、外、旁分泌功能是相适应的。Al^ract： Immunohistochemical localization and morphology of 5-Hydroxytryptamine immunoreactive cells （5-HT cell） in the digestive tract of Bombina orientalis have been investigated by using the ABC immunostaining technique and the origin of their distribution pattern as well as the relationship between cell morphology and function have been discussed .The 5-HT cells distribute throughout the digestive tract and their density is the highest in the cardiac part of stomach （55.73 ±10.67 number/mm^2 ）, followed by the pylorica part of stomach （42.99 ±5.25 number/mm^2 ） .The lowest cell density is found in rectum （6.37 ±4.78 number/mm^2 ）. The 5-HT cells have different shapes including round, ellipse, shuttle-like, tapering and other shapes. The majority of the 5-HT cells in esophagus and rectum are round or ellipse, but most of them are tapering in small intestine and the tapering cells have a long process extending to either the gut lumen or lamina propria. The 5-HT cells distribute at the basal region of epithelial cells, between epithelial cells, between glandular epithelial cells or in the lamina propria. In the cardiac part of stomach, body of stomach and pylorica part of stomach, most of them are between glandular epithelial cells and some are between epithelial cells. In bowel, the majority of 5-HT cells are between epithelial cells. The evidence indicates that the distribution pattern of 5-HT cells may be related to digestive function, feeding habit and food component and that the morphological characteristics of 5-HT cells are correlated with their endocrine, exocrine and paracrine functions.

关 键 词：东方铃蟾 5-HT细胞 免疫组织化学 

分 类 号：Q952[生物学—动物学]