低切应力对体外培养动脉壁细胞PDGF表达的影响  

Effects of Low Shear Stress on Expression of Platelet Derived Growth Factor(PDGF) of Organ-cultured Artery In Vitro

作　　者：刘波[1] 周瑾[2] 姜宗来[1] 张炎[3] 刘艳春[3] 严志强[1] 李玉泉[1] 

机构地区：[1]上海交通大学医学院生物力学实验室,上海200030 [2]第二军医大学生物化学与分子生物学教研室,上海200433 [3]第二军医大学解剖学教研室,上海200433

出　　处：《生物医学工程学杂志》2005年第1期5-9,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (10 13 2 0 2 0 ;10 0 72 0 75 ;3 0 10 0 0 60 )

摘　　要：为了研究切应力对完整血管的生物学作用 ,探讨应力引起血管重建过程中生长因子的变化 ,用血管应力培养系统体外培养猪颈总动脉 ,通过免疫组织化学、抗体夹心法酶联免疫吸附测定实验 (EL ISA)和计算机图像分析等方法 ,观察了低切应力作用下动脉壁细胞 PDGF- A、PDGF- B表达变化情况。结果显示 ,低切应力作用下体外培养动脉 EC中 PDGF- B及 VSMC中 PDGF- A的合成与分泌皆增加。PDGF表达上升 ,促进了 VSMC表型转换与增殖。在低切应力引起的血管重建中 ,这一机制可能起着重要作用。To explore the biological effects of shear stress on intact artery and the change of growth factor during stress-induced vascular remodeling, we stablished an artery organ-cultured system under stress in vitro, and the common carotid arteries of pigs were cultured under shear stress of 20, 5 and 0 dyn/cm^2. PDGF-A synthesis of vascular smooth muscle cells （VSMCs） cultured for 1, 4 and 7 days were studied by immunohistochemical and computer image processing methods, and PDGF-B secretion of endothelial cells （ECs） cultured within 12 h were studied by ELISA. Results showed that PDC-F-B increased obviously under shear stress of 5 dyn/cm^2, and reached the highest point at about 3 h; PDGF-A synthesis also obviously increased under low shear stress in 7 days. Increasing of PDGF synthesis promotes phenotype switch and proliferation of VSMC. It may have important influence on artery remodeling under low shear stress.

关 键 词：切应力 体外培养 细胞培养 动脉壁细胞 基因表达 血管重建 血小板衍生生长因子 血管平滑肌细胞 

分 类 号：R318.01[医药卫生—生物医学工程]